p21/CIP1/CDKN1A Antibody (CL13081) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44384

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13081

Format

Azide and BSA Free
View all p21/CIP1/CDKN1A Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P38936, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for p21/CIP1/CDKN1A Antibody (CL13081) - Azide and BSA Free

p21/CIP1/CDKN1A Antibody (CL13081) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody [NBP3-44384]

Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody [NBP3-44384]

Staining of A549 cells using the Anti-p21/CIP1/CDKN1A monoclonal antibody).
p21/CIP1/CDKN1A Antibody (CL13081) - Azide and BSA Free Western Blot: p21/CIP1/CDKN1A Antibody [NBP3-44384]

Western Blot: p21/CIP1/CDKN1A Antibody [NBP3-44384]

Analysis in human cell line RT-4.

Applications for p21/CIP1/CDKN1A Antibody (CL13081) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
For use with Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p21/CIP1/CDKN1A

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

Additional p21/CIP1/CDKN1A Products

Product Documents for p21/CIP1/CDKN1A Antibody (CL13081) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for p21/CIP1/CDKN1A Antibody (CL13081) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Cell Cycle

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Protocols

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FAQs

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