p21/CIP1/CDKN1A Antibody (DCS-60.2)

Novus Biologicals | Catalog # NBP2-44525

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative), Rat (Negative)

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Simple Western, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # DCS-60.2
View all p21/CIP1/CDKN1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human p21/CIP1/CDKN1A protein (Uniprot: P38936)

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Nuclear

Specificity

This monoclonal antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47781)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for p21/CIP1/CDKN1A Antibody (DCS-60.2)

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525]

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525]

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Human Bladder Carcinoma stained with p21/CIP1/CDKN1A Antibody (DCS-60.2).
Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525]

Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525]

Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Simple Western lane view shows a specific band for p21/CIP1/CDKN1A in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525]

Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525]

Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Electropherogram image of the corresponding Simple Western lane. p21/CIP1/CDKN1A antibody was used at 10 ug/ml dilution of MCF-7 lysates(s) respectively.
p21/CIP1/CDKN1A Antibody (DCS-60.2)

Western Blot: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] -

Western Blot: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (DCS-60.2).
p21/CIP1/CDKN1A Antibody (DCS-60.2)

Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] -

Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Lane view shows a specific band for p21/CIP1/CDKN1A using 2 mg/mL of MCF-7 cell lysate and 10 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view. Image reported by internal validation.

Applications for p21/CIP1/CDKN1A Antibody (DCS-60.2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

2-4 ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Use in Western Blot reported in scientific literature (PMID: 33086074).
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate(s), separated by Size, antibody dilution of 10 ug/mL, apparent MW was 28 kDa.

Reviewed Applications

Read 1 review rated 5 using NBP2-44525 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: p21/CIP1/CDKN1A

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

UniProt

P38936

Additional p21/CIP1/CDKN1A Products

Product Documents for p21/CIP1/CDKN1A Antibody (DCS-60.2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p21/CIP1/CDKN1A Antibody (DCS-60.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Cell Cycle

Citations for p21/CIP1/CDKN1A Antibody (DCS-60.2)

Customer Reviews for p21/CIP1/CDKN1A Antibody (DCS-60.2) (1)

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  • p21/CIP1/CDKN1A Antibody (DCS-60.2)
    Name: Tirthankar Ray
    Application: Western Blot
    Sample Tested: MCF10A whole cell lysate, Sample Amount: 30ug
    Species: Mouse
    Verified Customer | Posted 03/14/2017

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