Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # KIP1/1355R

Format

Azide and BSA Free
View all p27/Kip1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CDKN1B protein (Uniprot: P46527)

Localization

Cytoplasm, Endosome, Nucleus

Specificity

This antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free

p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free

Western Blot: p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free [NBP3-49592] -

Western blot analysis of A431 lysate using p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free.
p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free

Western Blot: p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free [NBP3-49592] -

Western Blot Analysis of NIH/3T3 cell lysate using p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free.
p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free [NBP3-49592] -

IHC analysis of formalin-fixed, paraffin-embedded human bladder. Strong nuclear staining using p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�C: HRP-polymer, 30min. DAB, 5min.

Applications for p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Cancer Biomarkers
Cell Cycle

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail