P2X3/P2RX3 Antibody

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• P2X3/P2RX3 Antibody

  Name: GUANGKUO DONG

  Application: Immunohistochemistry-Frozen

  Sample Tested: mouse tongue

  Species: Mouse

  Verified Customer | Posted 02/10/2020

  Mouse tongue, 20x

  Tissue: Frozen mouse tongue Postfixed in 4% PFA Cyroprotected in 20% sucrose Cryosectioned at 8 um Protocol: · Rinse sections with PBS 3 x 3 min · Fix in ice-cold acetone – 5 min · Rinse 3X3 PBS · Block with 2% donkey serum in PBS · Primary antibody – overnight at 4 deg C o P2X3 at 1:10, 1:50, 1:100 or negative control/no antibody o Mix= 0.05% Triton + 0.2% carrageenan in PBS · Rinse PBS 3x3 min · Secondary antibody: Incubate 1 hour at RT o Alexa fluor donkey anti-goat 488 1:500 o Mix=0.05% Triton + 0.2% carrageenan in PBS Protect from light · Rinse PBS 3X3 min · Counterstain with DAPI – 5 min at room temp · Rinse PBS 3x3 min

  
  Bio-Techne Response

  This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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