P2Y1/P2RY1 Antibody - BSA Free

Novus Biologicals | Catalog # NLS387

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Bat

Cited:

Rat

Predicted:

Avian (93%), Bovine (93%), Chicken (93%), Equine (93%), Guinea Pig (93%), Hamster (93%), Monkey (93%), Porcine (93%), Primate (100%), Rabbit (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all P2Y1/P2RY1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 15 amino acid peptide from 2nd extracellular domain of human P2Y1/P2RY1.

Epitope

2nd extracellular domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Mouse (87%), Rat (87%), Elephant (87%), Xenopus (87%).

Specificity

Human P2Y1/P2RY1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for P2Y1/P2RY1 Antibody - BSA Free

Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody - BSA Free [NLS387]

Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody - BSA Free [NLS387]

Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody [NLS387] - Analysis of anti-P2RY1 / P2Y1 antibody with human brain, neurons and glia.
P2Y1/P2RY1 Antibody - BSA Free

Immunohistochemistry: P2Y1/P2RY1 Antibody - BSA Free [NLS387] -

Immunohistochemistry: P2Y1/P2RY1 Antibody - BSA Free [NLS387] - Immunohistochemistry of Lung, adenocarcinoma

Applications for P2Y1/P2RY1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25683778)..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: P2Y1/P2RY1

P2Y1 is a Purinergic Receptor that causes a change in platelet shape and potentially leads to platelet aggregation by binding ADP, which results in the mobilization of intracellular calcium ions via activation of phospholipase C. This receptor also mediates the release of the relaxing factor nitric oxide and may be involved in the modulation of neuronal signal transmission. P2Y1 has been reported to be expressed in tissues throughout the body, particularly brain. ESTs have been isolated from human embryo, lung, and placenta libraries.

Long Name

P2Y Purinergic Receptor 1

Alternate Names

P2RY1

Entrez Gene IDs

5028 (Human)

Gene Symbol

P2RY1

UniProt

P47900 (Human)

Additional P2Y1/P2RY1 Products

Product Documents for P2Y1/P2RY1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for P2Y1/P2RY1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for P2Y1/P2RY1 Antibody - BSA Free

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Protocols

View specific protocols for P2Y1/P2RY1 Antibody - BSA Free (NLS387):

Immunohistochemistry Protocol for P2Y1 Antibody (NLS387):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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