P2Y2 Antibody (HL2801)
Novus Biologicals | Catalog # NBP3-48734
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2801
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Product Specifications
Immunogen
Synthetic peptide encompassing a sequence within the Extracellular domain mouse of P2Y2. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for P2Y2 Antibody (HL2801)
Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -
P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.
P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody (HL2801) [NBP3-48734] -
P2Y2 antibody [HL2801] detects P2Y2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -
P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat small intestine.
P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -
P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.
P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -
P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody (HL2801) [NBP3-48734] -
P2Y2 antibody [HL2801] detects P2Y2 protein by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:100.
Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -
P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for P2Y2 Antibody (HL2801)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: P2Y2/P2RY2
Long Name
P2Y Purinergic Receptor 2
Alternate Names
P2RU1, P2RY2, P2U, P2U1, P2Y2R
Gene Symbol
P2RY2
Additional P2Y2/P2RY2 Products
Product Documents for P2Y2 Antibody (HL2801)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for P2Y2 Antibody (HL2801)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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