P2Y2 Antibody (HL2801)

Novus Biologicals | Catalog # NBP3-48734

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2801
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Product Specifications

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain mouse of P2Y2. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for P2Y2 Antibody (HL2801)

P2Y2 Antibody (HL2801)

Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -

P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
P2Y2 Antibody (HL2801)

Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody (HL2801) [NBP3-48734] -

P2Y2 antibody [HL2801] detects P2Y2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:500.
Blue: Fluoroshield with DAPI.
P2Y2 Antibody (HL2801)

Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -

P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat small intestine.
P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
P2Y2 Antibody (HL2801)

Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -

P2Y2 antibody [HL2801] detects P2Y2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
P2Y2 Antibody (HL2801)

Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -

P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
P2Y2 Antibody (HL2801)

Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody (HL2801) [NBP3-48734] -

P2Y2 antibody [HL2801] detects P2Y2 protein by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:100.
Blue: Fluoroshield with DAPI.
P2Y2 Antibody (HL2801)

Immunohistochemistry-Paraffin: P2Y2 Antibody (HL2801) [NBP3-48734] -

P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (NBP3-48734) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for P2Y2 Antibody (HL2801)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P2Y2/P2RY2

P2Y2 belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to both adenosine and uridine nucleotides. It may participate in control of the cell cycle of endometrial carcinoma cells. P2Y2 is also a morphogen receptor that potentiates neurotrophin signaling in neuronal development and regeneration.

Long Name

P2Y Purinergic Receptor 2

Alternate Names

P2RU1, P2RY2, P2U, P2U1, P2Y2R

Gene Symbol

P2RY2

Additional P2Y2/P2RY2 Products

Product Documents for P2Y2 Antibody (HL2801)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for P2Y2 Antibody (HL2801)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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