p38 beta/MAPK11 Antibody (E13-Q)
Novus Biologicals | Catalog # NBP3-08206
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # E13-Q
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Product Specifications
Immunogen
Peptide derived from the C-terminus of human p38 beta/MAPK11 sequence. Antibody recognizes the epitope located between Glu352 - Glu363.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
Scientific Data Images for p38 beta/MAPK11 Antibody (E13-Q)
Western Blot: p38 beta/MAPK11 Antibody (E13-Q) [NBP3-08206]
Western Blot: p38 beta/MAPK11 Antibody (E13-Q) [NBP3-08206] - Western blot analysis of p38 beta/MAPK11. Lane A - human recombinant p38-alpha (260-360a.a., N-terminal GST tag; H00001432-Q01), lane B - human recombinant p38 beta/MAPK11 (with C-terminal DDK tag, NBL1-12870), lane C - human recombinant PKC-gamma (1-368a.a., N-terminal GST tag, H00006300-P01), lane D - human recombinant p38-delta (1-366a.a., N-terminal GST tag, H00005603-P01). 500ng of recombinant protein was loaded in each well.Immunocytochemistry/ Immunofluorescence: p38 beta/MAPK11 Antibody (E13-Q) [NBP3-08206]
Immunocytochemistry/Immunofluorescence: p38 beta/MAPK11 Antibody (E13-Q) [NBP3-08206] - Representative picture of p38 beta/MAPK11 expression in HEK293 cells, visualized with clonal rabbit anti-p38 beta/MAPK11 monospecific antibody. Primary antibody dilution - 1:500.Applications for p38 beta/MAPK11 Antibody (E13-Q)
Application
Recommended Usage
ELISA
1:10000-1:20000
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
20 mM Tris-HCl, pH 8.0, 10 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: p38 beta/MAPK11
Long Name
Mitogen-Activated Protein Kinase 11
Alternate Names
MAPK11, MPK11, p38b, p38beta, PRKM11, SAPK2B
Gene Symbol
MAPK11
Additional p38 beta/MAPK11 Products
Product Documents for p38 beta/MAPK11 Antibody (E13-Q)
Product Specific Notices for p38 beta/MAPK11 Antibody (E13-Q)
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Pathogen or Damage-activated C-Type Lectin Receptor Signaling Pathways
TGF-beta Signaling Pathways
Toll-Like Receptor Signaling Pathways
VEGF - VEGF R2 Signaling Pathways