p67phox/NOXA2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82542

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all p67phox Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFACEVLYNIAFMYAKKEEWKKAEEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIPVGRLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p67phox/NOXA2 Antibody - BSA Free

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Analysis in human bone marrow and skeletal muscle tissues. Corresponding p67phox/NOXA2 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: p67phox/NOXA2 Antibody [NBP1-82542]

Immunocytochemistry/ Immunofluorescence: p67phox/NOXA2 Antibody [NBP1-82542]

Immunocytochemistry/Immunofluorescence: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human cell line U-251 MG shows localization to nucleoli and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human lung shows strong cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human placenta shows moderate to strong cytoplasmic positivity in lymphoid cells.

Applications for p67phox/NOXA2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p67phox

NOXA2 encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in two transcript variants that encode the same protein.

Alternate Names

NCF2, NOXA2

Gene Symbol

NCF2

Additional p67phox Products

Product Documents for p67phox/NOXA2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for p67phox/NOXA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neutrophils

Citations for p67phox/NOXA2 Antibody - BSA Free

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Protocols

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