p73 Antibody (ER-15)

Novus Biologicals | Catalog # NB600-1279

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Gel Super Shift Assays

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ER-15
View all p73 Primary Antibodies »

Product Specifications

Immunogen

Purified monkey recombinant p73alpha protein (aa380-637)

Reactivity Notes

Human, Monkey and Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for p73 Antibody (ER-15)

Western Blot: p73 Antibody (ER-15) [NB600-1279]

Western Blot: p73 Antibody (ER-15) [NB600-1279]

Western Blot: p73 Antibody (ER-15) [NB600-1279] - Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using p73 alpha/beta Monoclonal Antibody, shows increased levels of p73 alpha/beta in MCF7 cell line upon doxorubicin treatment.
Western Blot: p73 Antibody (ER-15) [NB600-1279]

Western Blot: p73 Antibody (ER-15) [NB600-1279]

Western Blot: p73 Antibody (ER-15) [NB600-1279] - WB analysis was performed on nuclear enriched extracts (30 ug lysate) of MCF7 (Lane 1) and MCF7 treated with Doxorubicine (0.5 uM Doxorubicin for 24 hours) (Lane 2). The blot was probed with Anti-p73 alpha/beta Mouse Monoclonal Antibody.
Immunofluorescence: p73 Antibody (ER-15) [NB600-1279]

Immunofluorescence: p73 Antibody (ER-15) [NB600-1279]

Immunofluorescence: p73 Antibody (ER-15) [NB600-1279] - Analysis of p73 alpha/beta was performed using 70% confluent log phase MCF-7 cells.

Applications for p73 Antibody (ER-15)

Application
Recommended Usage

Gel Super Shift Assays

1:1 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4) and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p73

p73 protein is a member of the p53 family of proteins. The tumor-suppressor protein p53 exhibits sequence specific DNA binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage. In response to signals generated by a variety of genotoxic stresses, e.g, UV irradiation or DNA damage, p53 is expressed and undergoes post translational modification that results in its accumulation in the nucleus. Activation of p53 leads to cell cycle arrest and in some cases to apoptosis, resulting in the inability of genetically damaged cells to proliferate. Thus, the p53 dependent pathways help to maintain genomic stability by eliminating damaged cells. The accumulation of high levels of p53 is a potential marker for malignancy. p73 protein is expressed in either full length form (p73 alpha 80 kDa) or a shorter (p73 beta 70 kDa) mRNA variant. p73 gene was predicted to encode a protein with significant amino acid sequence similarity to p53. Each of the p53 amino acid residues implicated in direct sequence specific DNA binding is conserved in p73. p73 can activate the transcription of p53 responsive genes and inhibit cell growth in a p53 like manner by inducing apoptosis and cell cycle arrest. p73 has been mapped at chromosome 1p36.3, which is a locus of multiple suppressor genes for tumors including neuroblastoma and other cancers. c-Abl stimulates p73 mediated transactivation and apoptosis. This regulation of p73 by c-Abl in response to DNA damage was also demonstrated by a failure of ionizing radiation induced apoptosis after disruption of c-Abl-p73 interaction. The p73 protein can activate p53 responsive promoters and induce apoptosis in cells deficient in p53, which proves that inactivation of p53 family members may contribute to the biological properties of a subset of p53 mutants. The transcriptional activities of p53, p73 alpha, and p73 beta to induce their common cellular target genes differ among one another. Despite the high degree of similarity between p53 and p73, adenovirus E1B 55K, simian virus 40 T, and human papilloma virus E6 do not physically interact with p73, indicating that the functions of these two proteins may differ.

Long Name

Tumor Protein p73

Alternate Names

TP73

Entrez Gene IDs

7161 (Human)

Gene Symbol

TP73

UniProt

O15350 (Human)

Additional p73 Products

Product Documents for p73 Antibody (ER-15)

Certificate of Analysis

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Product Specific Notices for p73 Antibody (ER-15)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p73 Antibody (ER-15)

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Protocols

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