p97/VCP Antibody

Novus Biologicals | Catalog # NB100-1557

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (100%), Porcine (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all VCP Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).

Reactivity Notes

Rat reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p97/VCP Antibody

Western Blot: p97/VCP Antibody [NB100-1557]

Western Blot: p97/VCP Antibody [NB100-1557]

Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: p97/VCP Antibody [NB100-1557]

Immunohistochemistry-Paraffin: p97/VCP Antibody [NB100-1557]

Immunohistochemistry-Paraffin: p97/VCP Antibody [NB100-1557] - Sections of human ovarian carcinoma (upper) and mouse squamous cell carcinoma (lower). Antibody: Affinity purified rabbit anti-VCP used at a dilution of 1:200 (1ug/ml). Detection: DAB
p97/VCP Antibody [NB100-1557] - Detection of human and mouse VCP by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody NB100-1557 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
p97/VCP Antibody [NB100-1557] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1557 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1558. For blotting immunoprecipitated VCP, NB100-1557 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for p97/VCP Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200- 1:1000

Immunoprecipitation

1-5 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. p97/VCP antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-1557 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VCP

Valosin-containing protein (VCP) is a multifunctional protein that is proposed to be involved in vesicle transport and fusion for protein trafficking, 26S proteasome function in ubiquitin-based degradation pathways, and the assembly of peroxisomes. Mutations in the human VCP gene have been associated with diseases such as inclusion body myopathy, Paget disease of the bone, and frontotemporal dementia (IBMPFD). VCP can inhibit apoptosis and may play a role in tumorigenesis. VCP is also known as, Transitional Endoplasmic Reticulum ATPase, TER ATPase, TERA, 15S Mg (2+)-ATPase p97subunit, IBMPFD, Inclusion Body Myopathy associated with Paget Disease of Bone and Frontotemporal Dementia, MGC8560; MGC131997; MGC148092.

Long Name

Valosin-containing Protein

Alternate Names

IBMPFD, TERA

Entrez Gene IDs

7415 (Human); 67459 (Mouse)

Gene Symbol

VCP

UniProt

NP_009057.1 (Human)

Additional VCP Products

Product Documents for p97/VCP Antibody

Certificate of Analysis

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Product Specific Notices for p97/VCP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
NFkB Pathway

Citations for p97/VCP Antibody

Customer Reviews for p97/VCP Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
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4 Stars
0%
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2 Stars
0%
1 Stars
0%

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Customer Images

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  • p97/VCP Antibody
    Name: Luiz Alexandre Magno
    Application: Western Blot
    Sample Tested: PC12 cell whole cell lysate.
    Species: Rat
    Verified Customer | Posted 10/17/2018
    Ten PC12 cell lysate showing immunoreactivity against VCP antibody.
    It works very well at 1:1,000 dilution when loading gels with 30 ug of protein extracts (PC12 cells in my case).
    p97/VCP Antibody NB100-1557

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