![Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP2-15162]](https://resources.rndsystems.com/images/products/p97-VCP-Antibody-Immunohistochemistry-Paraffin-NBP2-15162-img0002.jpg "Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP2-15162]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Primate (100%), Xenopus (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human p97/VCP. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p97/VCP Antibody
Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP2-15162]
Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP2-15162] - Gastric cancer. VCP antibody [N3C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: p97/VCP Antibody [NBP2-15162]](https://resources.rndsystems.com/images/products/p97-VCP-Antibody-Western-Blot-NBP2-15162-img0003.jpg "Western Blot: p97/VCP Antibody [NBP2-15162]")
Western Blot: p97/VCP Antibody [NBP2-15162]
Western Blot: p97/VCP Antibody [NBP2-15162] - 50 ug Rat brain lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution: 1:10000.![Western Blot: p97/VCP Antibody [NBP2-15162]](https://resources.rndsystems.com/images/products/p97-VCP-Antibody-Western-Blot-NBP2-15162-img0004.jpg "Western Blot: p97/VCP Antibody [NBP2-15162]")
Western Blot: p97/VCP Antibody [NBP2-15162]
Western Blot: p97/VCP Antibody [NBP2-15162] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: p97/VCP Antibody [NBP2-15162] -
Western Blot: p97/VCP Antibody [NBP2-15162] - p97/VCP antibody detects p97/VCP protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p97/VCP antibody (NBP2-15162) diluted by 1:1000.Applications for p97/VCP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:20000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VCP
Long Name
Valosin-containing Protein
Alternate Names
IBMPFD, TERA
Gene Symbol
VCP
UniProt
Additional VCP Products
Product Documents for p97/VCP Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for p97/VCP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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