PA28 Activator beta Subunit/PSME2 Antibody (1G4) - Azide and BSA Free

Novus Biologicals | Catalog # H00005721-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G4

Format

Azide and BSA Free
View all PA28 Activator beta Subunit Primary Antibodies »

Product Specifications

Immunogen

PSME2 (NP_002809, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAKPCGVRLSGEARKQVEVFRQNLFQEAEEFLYRFLPQKIIYLNQLLQEDSLNVADLTSLRAPLDIPIPDPPPKDDEMETDKQEKKEVHK

Specificity

PSME2 - proteasome (prosome, macropain) activator subunit 2 (PA28 beta)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PA28 Activator beta Subunit/PSME2 Antibody (1G4) - Azide and BSA Free

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of PSME2 expression in transfected 293T cell line by PSME2 monoclonal antibody (M02), clone 1G4.Lane 1: PSME2 transfected lysate(27.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

Immunohistochemistry-Paraffin: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

Immunohistochemistry-Paraffin: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of monoclonal antibody to PSME2 on formalin-fixed paraffin-embedded human stomach carcinoma. Antibody concentration 6 ug/ml.
Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - PSME2 monoclonal antibody (M02), clone 1G4 Analysis of PSME2 expression in MCF-7.
Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Analysis of PSME2 transfected lysate using anti-PSME2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSME2 MaxPab rabbit polyclonal antibody.
ELISA: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

ELISA: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02]

ELISA: PA28 Activator beta Subunit/PSME2 Antibody (1G4) [H00005721-M02] - Detection limit for recombinant GST tagged PSME2 is approximately 0.3ng/ml as a capture antibody.

Applications for PA28 Activator beta Subunit/PSME2 Antibody (1G4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PA28 Activator beta Subunit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13.

Long Name

Proteasome (Prosome, Macropain) Activator Subunit 2

Alternate Names

MCP Activator, PSME2

Entrez Gene IDs

5721 (Human)

Gene Symbol

PSME2

OMIM

602161 (Human)

UniProt

NP_002809 (Human)

Additional PA28 Activator beta Subunit Products

Product Documents for PA28 Activator beta Subunit/PSME2 Antibody (1G4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PA28 Activator beta Subunit/PSME2 Antibody (1G4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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