PAK1/2/3 [p Ser144] Antibody (SR2102) - BSA Free

Novus Biologicals | Catalog # NBP3-21523

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2102 expressed in HEK293

Format

BSA Free
View all PAK1/2/3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-PAK1/2/3 [Ser144/Ser141/Ser139] (Uniprot #: O75914, Q13153, Q13177)

Modification

p Ser 144

Specificity

Detects endogenous levels of total phospho-PAK1/2/3 [Ser144/Ser141/Ser139]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAK1/2/3 [p Ser144] Antibody (SR2102) - BSA Free

Immunohistochemistry: PAK1/2/3 [p Ser 144] Antibody (SR2102) [NBP3-21523] -

Immunohistochemistry: PAK1/2/3 [p Ser 144] Antibody (SR2102) [NBP3-21523] - Immunohistochemical analysis of paraffin-embedded mouse brain, using NBP3-21523.

Western Blot: PAK1/2/3 [p Ser 144] Antibody (SR2102) [NBP3-21523] -

Western Blot: PAK1/2/3 [p Ser 144] Antibody (SR2102) [NBP3-21523] - Western blot analysis using NBP3-21523 in HeLa Cell lysate treated with lambda phosphatase.

Applications for PAK1/2/3 [p Ser144] Antibody (SR2102) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:1000-1:10000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAK1/2/3

In mammals, there are several identified isoforms of p21-activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously. Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death. Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK. (1-4)

Alternate Names

Alpha-PAK, EC 2.7.11, EC 2.7.11.1, MGC130000, MGC130001, p21 protein (Cdc42/Rac)-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related), p21-activated kinase 1, p65-PAK, PAK-1, PAKalpha, serine/threonine-protein kinase PAK 1, STE20 homolog, yeast

Gene Symbol

PAK1

Additional PAK1/2/3 Products

Product Documents for PAK1/2/3 [p Ser144] Antibody (SR2102) - BSA Free

Certificate of Analysis

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Product Specific Notices for PAK1/2/3 [p Ser144] Antibody (SR2102) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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