PAK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85802

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAK1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NSQKYMSFTDKSAEDYNSSNALNVKAVSETPAVPPVSEDEDDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23436904)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK1 Antibody - BSA Free

PAK1 Antibody - BSA Free Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Staining of human rectum shows strong cytoplasmic ad membranous positivity in glandular cells.
PAK1 Antibody - BSA Free Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Staining of human pancreas shows no positivity in endocrine glandular cells as expected.
PAK1 Antibody - BSA Free Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.
PAK1 Antibody - BSA Free Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
PAK1 Antibody - BSA Free Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP1-85802]

Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using HPA003565 antibody. Corresponding PAK1 RNA-seq data are presented for the same tissues.
PAK1 Antibody - BSA Free Western Blot: PAK1 Antibody - BSA Free [NBP1-85802]

Western Blot: PAK1 Antibody - BSA Free [NBP1-85802]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
PAK1 Antibody - BSA Free Western Blot: PAK1 Antibody - BSA Free [NBP1-85802]

Western Blot: PAK1 Antibody - BSA Free [NBP1-85802]

Analysis in human cell lines U2OS and MCF-7 using Anti-PAK1 antibody. Corresponding PAK1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
PAK1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: PAK1 Antibody - BSA Free [NBP1-85802]

Immunocytochemistry/ Immunofluorescence: PAK1 Antibody - BSA Free [NBP1-85802]

Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Applications for PAK1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAK1

p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).

Long Name

p21/Cdc42/Rac1-activated Kinase 1

Alternate Names

alpha-PAK

Gene Symbol

PAK1

Additional PAK1 Products

Product Documents for PAK1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PAK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
PDGF Family

Citations for PAK1 Antibody - BSA Free

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Protocols

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