PAK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19680

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAK1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (84%), Xenopus Laevis (84%).

Localization

Cytoplasm; Cell junction; focal adhesion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PAK1 Antibody - BSA Free (NBP2-19680) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PAK1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680]

Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680]

Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680] - Paraffin-embedded mouse fore brain. PAK1 antibody [N2C2], Internal dilution: 1:500.
Western Blot: PAK1 Antibody [NBP2-19680]

Western Blot: PAK1 Antibody [NBP2-19680]

Western Blot: PAK1 Antibody [NBP2-19680] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: PAK1 Antibody [NBP2-19680]

Western Blot: PAK1 Antibody [NBP2-19680]

Western Blot: PAK1 Antibody [NBP2-19680] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680]

Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680]

Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680] - HBL435 xenograft.
PAK1 Antibody - BSA Free

Western Blot: PAK1 Antibody [NBP2-19680] -

PAK1 antibody detects PAK1 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK1 antibody (NBP2-19680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PAK1 Antibody - BSA Free

Western Blot: PAK1 Antibody [NBP2-19680] -

PAK1 antibody [N2C2], Internal detects PAK1 protein by western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
7.5% SDS-PAGE
PAK1 antibody [N2C2], Internal (NBP2-19680) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PAK1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAK1

PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

p21/Cdc42/Rac1-activated Kinase 1

Alternate Names

alpha-PAK

Gene Symbol

PAK1

UniProt

Q13153

Additional PAK1 Products

Product Documents for PAK1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PAK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Intracellular Kinases
PDGF Family

Customer Reviews for PAK1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review PAK1 Antibody - BSA Free and earn rewards!

Have you used PAK1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies