PANK2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88295

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PANK2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLHTVFCATGGGAYKFEQDFLTIGDLQLCKLDELDCLIKGILYIDSVGFNGRSQCYYFENPADSEKCQKLPFDLKNPYPLLLVNIGSGVSILAVYSKDNYKRVTGT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PANK2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PANK2 Antibody [NBP1-88295]

Immunocytochemistry/ Immunofluorescence: PANK2 Antibody [NBP1-88295]

Immunocytochemistry/Immunofluorescence: PANK2 Antibody [NBP1-88295] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295]

Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295]

Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295]

Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295]

Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295]

Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295]

Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295]

Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295]

Immunohistochemistry-Paraffin: PANK2 Antibody [NBP1-88295] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
PANK2 Antibody - BSA Free Western Blot: PANK2 Antibody - BSA Free [NBP1-88295]

Western Blot: PANK2 Antibody - BSA Free [NBP1-88295]

Analysis in control (vector only transfected HEK293T lysate) and PANK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for PANK2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:10-1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PANK2

PANK2 encodes a protein belonging to the pantothenate kinase family and is the only member of that family to be expressed in mitochondria. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by acyl CoA species. Mutations in this gene are associated with HARP syndrome and pantothenate kinase-associated neurodegeneration (PKAN), formerly Hallervorden-Spatz syndrome. Alternative splicing, involving the use of alternate first exons, results in multiple transcripts encoding different isoforms.

Long Name

Pantothenate Kinase 2

Alternate Names

FLJ11729, HPANK2, NBIA1, PKAN

Gene Symbol

PANK2

Additional PANK2 Products

Product Documents for PANK2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PANK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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FAQs

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