Papilin Antibody (5B2E5) - BSA Free
Novus Biologicals | Catalog # NBP2-37320
Loading...
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5B2E5
Format
BSA Free
Loading...
Product Specifications
Immunogen
Purified recombinant fragment of human Papilin (AA: 766-870) expressed in E. Coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
137.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Papilin Antibody (5B2E5) - BSA Free
Western Blot: Papilin Antibody (5B2E5)BSA Free [NBP2-37320]
Western Blot: Papilin Antibody (5B2E5) [NBP2-37320] - Western blot analysis using PAPLN mouse mAb against Hela (1), HepG2 (2), OCM-1 (3), Raji (4), Jurkat (5), NIH/3T3 (6) cell lysate.Immunocytochemistry/ Immunofluorescence: Papilin Antibody (5B2E5) - BSA Free [NBP2-37320]
Immunocytochemistry/Immunofluorescence: Papilin Antibody (5B2E5) [NBP2-37320] - Immunofluorescence analysis of NIH/3T3 cells using PAPLN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Western Blot: Papilin Antibody (5B2E5)BSA Free [NBP2-37320]
Western Blot: Papilin Antibody (5B2E5) [NBP2-37320] - Western blot analysis using PAPLN mAb against human PAPLN recombinant protein. (Expected MW is 36.4 kDa)Western Blot: Papilin Antibody (5B2E5)BSA Free [NBP2-37320]
Western Blot: Papilin Antibody (5B2E5) [NBP2-37320] - Western blot analysis using PAPLN mAb against HEK293 (1) and PAPLN (AA: 766-870)-hIgGFc transfected HEK293 (2) cell lysate.ELISA: Papilin Antibody (5B2E5) - BSA Free [NBP2-37320]
ELISA: Papilin Antibody (5B2E5) [NBP2-37320] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Applications for Papilin Antibody (5B2E5) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Papilin
Alternate Names
PAPLN
Gene Symbol
PAPLN
Additional Papilin Products
Product Documents for Papilin Antibody (5B2E5) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Papilin Antibody (5B2E5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Papilin Antibody (5B2E5) - BSA Free
Customer Reviews for Papilin Antibody (5B2E5) - BSA Free
There are currently no reviews for this product. Be the first to review Papilin Antibody (5B2E5) - BSA Free and earn rewards!
Have you used Papilin Antibody (5B2E5) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...