PAPOLA Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87345

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAPOLA Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVK

Reactivity Notes

Mouse (88%), Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAPOLA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PAPOLA Antibody [NBP1-87345]

Immunocytochemistry/ Immunofluorescence: PAPOLA Antibody [NBP1-87345]

Immunocytochemistry/Immunofluorescence: PAPOLA Antibody [NBP1-87345] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PAPOLA Antibody [NBP1-87345]

Immunohistochemistry-Paraffin: PAPOLA Antibody [NBP1-87345]

Immunohistochemistry-Paraffin: PAPOLA Antibody [NBP1-87345] - Staining of human prostate shows moderate nuclear positivity in glandular cells.

Applications for PAPOLA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAPOLA

PAPOLA is a polymerase that creates the 3' poly(A) tail of mRNA's. It is also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. PAPOLA may acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.

Long Name

Poly(A) polymerase alpha

Alternate Names

EC 2.7.7.19, PAP, PAP-alpha

Gene Symbol

PAPOLA

Additional PAPOLA Products

Product Documents for PAPOLA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PAPOLA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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