Pappalysin-1/PAPP-A Antibody (9) - BSA Free
Novus Biologicals | Catalog # NBP1-05116
![Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]](https://resources.rndsystems.com/images/products/Pappalysin-1-PAPP-A-Antibody-NA-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05116-img0002.jpg "Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 9
Format
BSA Free
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Product Specifications
Immunogen
Purified human PAPP-A
Epitope
The epitope is located on the PAPP-A subunit of the heterotetramer.
Specificity
Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Pappalysin-1/PAPP-A Antibody (9) - BSA Free
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in paraffin embedded human placenta tissue.![Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]](https://resources.rndsystems.com/images/products/Pappalysin-1-PAPP-A-Antibody-NA-BSA-Free-Immunohistochemistry-Frozen-NBP1-05116-img0001.jpg "Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]")
Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]
Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in frozen human placenta tissue.Applications for Pappalysin-1/PAPP-A Antibody (9) - BSA Free
Application
Recommended Usage
ELISA
1:8000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: In sandwich ELISA the optimal combination is NBP1-05116 used as coating antibody and biotinylated NBP1-05043 used as detection antibody. NBP1-05116 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat.
WB: NBP1-05116 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of NBP2-21614 and NBP1-05043.
IHC: NBP1-05116 can be used in IHC.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Pappalysin-1/PAPP-A
Long Name
Pregnancy-associated Plasma Protein 1
Alternate Names
ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA, Pappalysin1
Gene Symbol
PAPPA
Additional Pappalysin-1/PAPP-A Products
Product Documents for Pappalysin-1/PAPP-A Antibody (9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pappalysin-1/PAPP-A Antibody (9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Pappalysin-1/PAPP-A Antibody (9) - BSA Free
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Q: I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).
A: Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.
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