Pappalysin-1/PAPP-A Antibody (9) - BSA Free

Novus Biologicals | Catalog # NBP1-05116

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 9

Format

BSA Free
View all Pappalysin-1/PAPP-A Primary Antibodies »

Product Specifications

Immunogen

Purified human PAPP-A

Epitope

The epitope is located on the PAPP-A subunit of the heterotetramer.

Specificity

Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]

Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]

Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in paraffin embedded human placenta tissue.
Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]

Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]

Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in frozen human placenta tissue.

Applications for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

Application
Recommended Usage

ELISA

1:8000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
ELISA: In sandwich ELISA the optimal combination is NBP1-05116 used as coating antibody and biotinylated NBP1-05043 used as detection antibody. NBP1-05116 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. WB: NBP1-05116 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of NBP2-21614 and NBP1-05043. IHC: NBP1-05116 can be used in IHC.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Pappalysin-1/PAPP-A

Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down's syndrome (trisomy 21).

Long Name

Pregnancy-associated Plasma Protein 1

Alternate Names

ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA, Pappalysin1

Gene Symbol

PAPPA

Additional Pappalysin-1/PAPP-A Products

Product Documents for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

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  • Q: I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).

    A: Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.

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