Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free
Novus Biologicals | Catalog # NBP2-21614
![Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free [NBP2-21614]](https://resources.rndsystems.com/images/products/Pappalysin-1-PAPP-A-Antibody-PAPP-A-1-1-BSA-Free-Immunohistochemistry-Paraffin-NBP2-21614-img0001.jpg "Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free [NBP2-21614]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PAPP A 1-1
Format
BSA Free
Loading...
Product Specifications
Immunogen
Purified human PAPP-A
Epitope
The epitope is located on the PAPP-A subunit of the heterotetramer.
Specificity
Specific for native human PAPP-A.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Concentration for any given batch will be between 0.85 - 1.15 mg/mL.
Scientific Data Images for Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free [NBP2-21614]
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free [NBP2-21614] - Human placenta tissue.![Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free [NBP2-21614]](https://resources.rndsystems.com/images/products/Pappalysin-1-PAPP-A-Antibody-PAPP-A-1-1-BSA-Free-Immunohistochemistry-Paraffin-NBP2-21614-img0002.jpg "Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free [NBP2-21614]")
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free [NBP2-21614]
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free [NBP2-21614] - staining of a formalin-fixed, paraffin-embedded placental tissue with pappalysin-1/PAPP-A Antibody.Applications for Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: In sandwich ELISA with a polyclonal antibody in coat, NBP2-21614 only reacts poorly with PAPP-A in third trimester serum.
WB: NBP2-21614 only detects unreduced PAPP-A in Western blotting.
IHC: NBP2-21614 can be used in IHC. Please consult www.proteinatlas.org
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Pappalysin-1/PAPP-A
Long Name
Pregnancy-associated Plasma Protein 1
Alternate Names
ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA, Pappalysin1
Gene Symbol
PAPPA
Additional Pappalysin-1/PAPP-A Products
Product Documents for Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free
There are currently no reviews for this product. Be the first to review Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free and earn rewards!
Have you used Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Pappalysin-1/PAPP-A Antibody (PAPP A 1-1) - BSA Free
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).
A: Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.
Loading...