Prostaglandin E2 (PGE2) is a prostanoid fatty acid derivative of arachidonic acid whose synthesis can be blocked by corticosteroids or by nonsteroidal anti-inflammatory drugs (NSAIDs). It is produced by a wide variety of tissues and is elevated in many bodily fluids during inflammation. PGE2 interacts with EP1, 2, 3, and 4 receptors to induce a complex range of effects. In the kidney, this includes stimulation of renin release and both promotion and inhibition of salt and water absorption. PGE2 can promote either smooth muscle relaxation or contraction. PGE2 is important in normal joint physiology and is a principal mediator of the inflammatory response to tissue damage. PGE2 sensitizes peripheral nociceptive nerves and exaggerates inflammatory and neuropathic pain by direct inhibition of spinal cord glycinergic neurotransmission. PGE2 also plays a role in hippocampal synaptic plasticity and febrile responses. PGE2 activity is linked to the synthesis and release of several hormones and the fetal hypothalamus-pituitary-adrenal axis. PGE2 also stimulates tumor cell proliferation and differentiation as well as tumor-associated neovascularization.
Parameter Immunoassay Control Set 777 Human Prostaglandin E2
R&D Systems | Catalog # QC142
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Product Summary for Parameter Immunoassay Control Set 777 Human Prostaglandin E2
INTENDED USE & DESCRIPTION
For use as quantitative controls for the determination of cytokine concentrations in biological fluids. Concentrations have been assigned using R&D Systems’ Parameter® kits. Controls are prepared in diluted porcine serum with preservatives. They contain recombinant human cytokines at low, medium and high concentrations. Controls are supplied lyophilized.
STORAGE & STABILITY
Unreconstituted Controls should be stored at 2-8 °C and are stable for at least 6 months from date of receipt. Depending on the analyte of interest, reconstituted controls may be stable when stored at < -20 °C. Users should evaluate the frozen stability of the controls in their application or discard after use.
REAGENT PREPARATION
Reconstitute each vial with the volume of deionized or distilled water indicated on the product datasheet.
PROCEDURE & EXPECTED VALUES
Controls should be assayed in the same manner as unknown specimens.
The acceptable ranges for the analytes in these controls are printed on the product datasheet. Due to possible variations in techniques and methodologies, it is recommended that each laboratory determine its own target range. Laboratories using other test systems should establish their own acceptable ranges as these assays may produce different values.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
• The ranges were determined using R&D Systems’ Parameter kits. If expected values are not obtained, verify that the lot numbers on the vials correspond with the lot numbers listed above and the correct volume of deionized or distilled water was used for reconstitution of the controls.
• The results obtained with these controls depend upon several factors associated with methods and instrumentation. Test systems other than those supplied by R&D Systems may result in values that differ from those printed on this product datasheet.
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Background: Prostaglandin E2/PGE2
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Product Documents for Parameter Immunoassay Control Set 777 Human Prostaglandin E2
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Product Specific Notices for Parameter Immunoassay Control Set 777 Human Prostaglandin E2
For research use only
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