Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human, Mouse, Avian - Chicken

Predicted:

Bovine (100%), Golden Syrian Hamster (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, In vitro assay

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Park7/DJ-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to the C-terminus (within residues 150-189) of human Park7(DJ-1). [UniProt# Q99497]

Reactivity Notes

Predicted to react with bovine, Zebra fish, and Golden Syrian hamster based on 100% sequence homology. Chicken reactivity reported in scientific literature (PMID: 24064392). Rat reactivity reported in scientific literature (PMID: 26419955)

Localization

Nucleus. Cytoplasm. Associated with mitochondria in some cells, particularly after oxidative stress. Detected in tau inclusions in brains from neurodegenerative disease patients.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Park7/DJ-1 Antibody - BSA Free (NB300-270) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF, Simple Western and IP. Anti-Park7/DJ-1 Antibody: Cited in 22 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Park7/DJ-1 Antibody - BSA Free

Simple Western: Park7/DJ-1 Antibody [NB300-270]

Simple Western: Park7/DJ-1 Antibody [NB300-270]

Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: Park7/DJ-1 Antibody [NB300-270]

Western Blot: Park7/DJ-1 Antibody [NB300-270]

Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0014.jpg
Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody [NB300-270]

Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody [NB300-270]

Park7-DJ-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-270-img0015.jpg
Western Blot: Park7/DJ-1 Antibody [NB300-270]

Western Blot: Park7/DJ-1 Antibody [NB300-270]

Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0013.jpg
Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB300-270]

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB300-270]

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB300-270] - Park7(DJ-1) detected in paraffin embedded human cortex.
Western Blot: Park7/DJ-1 Antibody [NB300-270]

Western Blot: Park7/DJ-1 Antibody [NB300-270]

Western Blot: Park7/DJ-1 Antibody [NB300-270] - Human HEK293 cells and mouse brain lysates.
Western Blot: Park7/DJ-1 Antibody [NB300-270]

Western Blot: Park7/DJ-1 Antibody [NB300-270]

Western Blot: Park7/DJ-1 Antibody [NB300-270] - Cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY PARK7 cDNA (lane 2) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Park7(DJ-1).
Park7/DJ-1 Antibody Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB300-270]

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB300-270]

Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Park7/DJ-1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Park7/DJ-1 Antibody

Western Blot: Park7/DJ-1 Antibody [NB300-270] -

Western Blot: Park7/DJ-1 Antibody [NB300-270] - Presence of oxDJ-1 in RPE cells subjected to oxidative stress.ARPE-19 monolayers were treated with increasing concentrations (0 to 800 µM) of H2O2 for 1 hr (A) & 18 hs (B), harvested, & analyzed by immunoblot assay with oxDJ-1 antibody (upper panel). Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). Each lane contained 20 µg of protein. A dose response is observed when cells are exposed to increasing concentrations of H2O2 for 1 h (A, lanes 1 to 6) & 18 hrs (B, lanes 7 to 12). Confocal immunofluorescence staining of baseline ARPE-19 cultures (C) fixed before extraction with Triton X-100 & labeling with oxDJ-1 antibodies revealed absence of oxDJ-1. However, oxDJ-1 is observed in the cytoplasm (arrows) & perinuclear area (arrowheads) of RPE cells exposure to 400 µM H2O2 for 1 h (D) & 18 hrs (E). Cell nuclei were labeled with TO-PRO-3. Scale bar = 20 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23844142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Park7/DJ-1 Antibody

Western Blot: Park7/DJ-1 Antibody [NB300-270] -

Western Blot: Park7/DJ-1 Antibody [NB300-270] - Presence of oxDJ-1 in RPE cells subjected to oxidative stress.ARPE-19 monolayers were treated with increasing concentrations (0 to 800 µM) of H2O2 for 1 hr (A) & 18 hs (B), harvested, & analyzed by immunoblot assay with oxDJ-1 antibody (upper panel). Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). Each lane contained 20 µg of protein. A dose response is observed when cells are exposed to increasing concentrations of H2O2 for 1 h (A, lanes 1 to 6) & 18 hrs (B, lanes 7 to 12). Confocal immunofluorescence staining of baseline ARPE-19 cultures (C) fixed before extraction with Triton X-100 & labeling with oxDJ-1 antibodies revealed absence of oxDJ-1. However, oxDJ-1 is observed in the cytoplasm (arrows) & perinuclear area (arrowheads) of RPE cells exposure to 400 µM H2O2 for 1 h (D) & 18 hrs (E). Cell nuclei were labeled with TO-PRO-3. Scale bar = 20 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23844142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Park7/DJ-1 Antibody

Western Blot: Park7/DJ-1 Antibody [NB300-270] -

Western Blot: Park7/DJ-1 Antibody [NB300-270] - Presence of oxDJ-1 in RPE cells subjected to oxidative stress.ARPE-19 monolayers were treated with increasing concentrations (0 to 800 µM) of H2O2 for 1 hr (A) & 18 hs (B), harvested, & analyzed by immunoblot assay with oxDJ-1 antibody (upper panel). Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). Each lane contained 20 µg of protein. A dose response is observed when cells are exposed to increasing concentrations of H2O2 for 1 h (A, lanes 1 to 6) & 18 hrs (B, lanes 7 to 12). Confocal immunofluorescence staining of baseline ARPE-19 cultures (C) fixed before extraction with Triton X-100 & labeling with oxDJ-1 antibodies revealed absence of oxDJ-1. However, oxDJ-1 is observed in the cytoplasm (arrows) & perinuclear area (arrowheads) of RPE cells exposure to 400 µM H2O2 for 1 h (D) & 18 hrs (E). Cell nuclei were labeled with TO-PRO-3. Scale bar = 20 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23844142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Park7/DJ-1 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 21937704)

Immunocytochemistry/ Immunofluorescence

1:500

Immunoprecipitation

1:200

In vitro assay

reported in scientific literature (PMID 21645620)

Simple Western

1:4000

Western Blot

1:2000
Application Notes

In Western Blot, a band can be seen at approx. 25 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.05 mg/mL, separated by Size, antibody dilution of 1:4000, apparent MW was 26 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 3 reviews rated 3.7 using NB300-270 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Park7/DJ-1

Park7/DJ-1 is one of several genes thought to cause autosomal-recessive early-onset parkinson's disease. It also acts as a positive regulator of androgen receptor-dependent transcription, as well as functions as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death.

Long Name

Parkinson Disease 7

Alternate Names

DJ-1, DJ1

Entrez Gene IDs

11315 (Human); 57320 (Mouse)

Gene Symbol

PARK7

UniProt

Q99497

Additional Park7/DJ-1 Products

Product Documents for Park7/DJ-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Park7/DJ-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Park7/DJ-1 Antibody - BSA Free

Customer Reviews for Park7/DJ-1 Antibody - BSA Free (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Park7/DJ-1 Antibody
    Name: Agnès Tessier
    Application: Simple Western
    Sample Tested: Human foreskin, adult ski, engineered human skin, keratinocytes, HaCaT cells
    Species: Human
    Verified Customer | Posted 06/17/2019
    Detection of Park7/DJ-1 by Simple Western (JESS, chemiluminescence) in reconstructed human epidermis lysate (about 600 µg/mL protein concentration). Antibody dilution: 1/20
    Park7/DJ-1 Antibody - BSA Free NB300-270
  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 09/10/2013
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa whole cell lysate, mouse brain
    Species: Human
    Verified Customer | Posted 12/19/2011
    Park7/DJ-1 Antibody - BSA Free NB300-270

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Protocols

View specific protocols for Park7/DJ-1 Antibody - BSA Free (NB300-270):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 10% formalin to the dish and fix at room temperature for 10 minutes.
2. Remove the formalin and wash the cells in PBS.
3. Permeablize the cells with 0.1% Triton X100 or other suitable detergent for 10 min.
4. Remove the permeablization buffer and wash three times for 10 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 10 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 10 minutes each.
10. Counter stain DNA with DAPi if required.

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

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