Parvalbumin Antibody (3C9) - BSA Free
Novus Biologicals | Catalog # NBP2-50038
![Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038]](https://resources.rndsystems.com/images/products/Parvalbumin-Antibody-3C9-Western-Blot-NBP2-50038-img0005.jpg "Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Equine
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3C9
Format
BSA Free
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Product Specifications
Immunogen
Full-length recombinant human Parvalbumin expressed in and purified from E. coli. [UniProt# P20472]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Parvalbumin Antibody (3C9) - BSA Free
Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038]
Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse parvalbumin mAb, dilution 1:1,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the parvalbumin antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.![Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]](https://resources.rndsystems.com/images/products/Parvalbumin-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP2-50038-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]")
Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]
Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of rat cerebellum section stained with mouse mAb to parvalbumin, NBP2-50038, dilution 1:1,000, in green, and costained with chicken pAb to calbindin, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear are green.![Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]](https://resources.rndsystems.com/images/products/Parvalbumin-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP2-50038-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]")
Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038]
Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Adult rat cerebellum floating section was stained with NBP2-50038 at 1:1,000 (green). Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue is a DNA stain.Applications for Parvalbumin Antibody (3C9) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:1000 - 1:5000
Application Notes
In WB, this antibody recognizes a band at 12 kDa in skeletal muscle lysates, which represents Parvalbumin. Note, this antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50% PBS, 50% glycerol
Format
BSA Free
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Parvalbumin
Alternate Names
D22S749, MGC116759, parvalbumin, parvalbumin alpha, PV
Gene Symbol
PVALB
UniProt
Additional Parvalbumin Products
Product Documents for Parvalbumin Antibody (3C9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Parvalbumin Antibody (3C9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Parvalbumin Antibody (3C9) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Parvalbumin Antibody (3C9) - BSA Free
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Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
A:
We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.
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