Patched 1/PTCH Antibody

Novus Biologicals | Catalog # NB100-2451

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Patched 1/PTCH Primary Antibodies »

Product Specifications

Immunogen

This Patched 1/PTCH Antibody was developed against a peptide with sequence ELQDVECEERPR corresponding to C-Terminus according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.

Specificity

This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Patched 1/PTCH Antibody

Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]

Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]

Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]

Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]

Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451] - Staining of paraffin embedded Human Cortex. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Patched 1/PTCH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

8 ug/mL

Immunohistochemistry-Paraffin

8 ug/mL

Peptide ELISA

Detection limit 1:16000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Patched 1/PTCH

Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas.

Alternate Names

BCNS, HPE7, mes, NBCCS, ptc1, PTCH1

Entrez Gene IDs

5727 (Human)

Gene Symbol

PTCH1

UniProt

NP_000255.1 (Human)

Additional Patched 1/PTCH Products

Product Documents for Patched 1/PTCH Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Patched 1/PTCH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Patched 1/PTCH Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Patched 1/PTCH Antibody

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  • Q: I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?

    A:

    NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.

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