Pax5/BSAP Antibody (CL14550) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44317

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL14550

Format

Azide and BSA Free
View all Pax5/BSAP Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q02548, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Pax5/BSAP Antibody (CL14550) - Azide and BSA Free

Pax5/BSAP Antibody (CL14550) - Azide and BSA Free Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Analysis in human lymph node and liver tissues using NBP3-44317 antibody. Corresponding Pax5/BSAP RNA-seq data are presented for the same tissues.
Pax5/BSAP Antibody (CL14550) - Azide and BSA Free Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Staining of human liver shows no nuclear positivity in hepatocytes as expected.
Pax5/BSAP Antibody (CL14550) - Azide and BSA Free Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Staining of human chronic lymphocytic leukemia shows moderate nuclear positivity in tumor cells.
Pax5/BSAP Antibody (CL14550) - Azide and BSA Free Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Staining of human kidney shows no nuclear positivity in cells in tubules as expected.
Pax5/BSAP Antibody (CL14550) - Azide and BSA Free Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP3-44317]

Staining of human lymph node shows weak to moderate nuclear positivity in lymphoid cells.
Pax5/BSAP Antibody (CL14550) - Azide and BSA Free Western Blot: Pax5/BSAP Antibody [NBP3-44317]

Western Blot: Pax5/BSAP Antibody [NBP3-44317]

Analysis in human cell line Daudi.

Applications for Pax5/BSAP Antibody (CL14550) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Pax5/BSAP

PAX5 encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq]

Long Name

Paired Box Gene 5/B-cell Specific Activator Protein

Alternate Names

BSAP

Gene Symbol

PAX5

Additional Pax5/BSAP Products

Product Documents for Pax5/BSAP Antibody (CL14550) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Pax5/BSAP Antibody (CL14550) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pax5/BSAP Antibody (CL14550) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Pax5/BSAP Antibody (CL14550) - Azide and BSA Free and earn rewards!

Have you used Pax5/BSAP Antibody (CL14550) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies