PBEF/Visfatin/NAMPT Antibody

Novus Biologicals | Catalog # NBP2-19468

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (96%), Chicken (96%), Mouse (96%), Porcine (97%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PBEF/Visfatin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PBEF/Visfatin/NAMPT. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (92%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PBEF/Visfatin/NAMPT Antibody

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NBP2-19468]

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NBP2-19468]

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NBP2-19468] - Paraffin-embedded rat liver. Visfatin antibody [N1N3] diluted at 1:500.
Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-19468]

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-19468]

Western Blot: NAMPT Antibody [NBP2-19468] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.
PBEF/Visfatin/NAMPT Antibody

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-19468] -

Visfatin antibody detects Visfatin protein by western blot analysis.
A. 30 ug A549 whole cell lysate/extract
B. 30 ug HCT116 whole cell lysate/extract
C. 30 ug MCF-7 whole cell lysate/extract
7.5 % SDS-PAGE
Visfatin antibody (NBP2-19468) dilution: 1:1000
PBEF/Visfatin/NAMPT Antibody

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-19468] -

Visfatin antibody detects Visfatin protein by western blot analysis.
A. 30 ug Jurkat whole cell lysate/extract
B. 30 ug Raji whole cell lysate/extract
C. 30 ug K562 whole cell lysate/extract
D. 30 ug NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
Visfatin antibody (NBP2-19468) dilution: 1:1000

Applications for PBEF/Visfatin/NAMPT Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PBEF/Visfatin

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq]

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Gene Symbol

NAMPT

UniProt

P43490

Additional PBEF/Visfatin Products

Product Documents for PBEF/Visfatin/NAMPT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PBEF/Visfatin/NAMPT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail