PBX1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19713

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Chicken (100%), Mouse (100%), Rat (100%), Xenopus (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PBX1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PBX1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (98%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PBX1 Antibody - BSA Free

Western Blot: PBX1 Antibody [NBP2-19713]

Western Blot: PBX1 Antibody [NBP2-19713]

Western Blot: PBX1 Antibody [NBP2-19713] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBX1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PBX1 Antibody [NBP2-19713]

Immunocytochemistry/ Immunofluorescence: PBX1 Antibody [NBP2-19713]

Immunocytochemistry/Immunofluorescence: PBX1 Antibody [NBP2-19713] - PBX1 antibody detects PBX1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PBX1 stained by PBX1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.
PBX1 Antibody - BSA Free

Immunoprecipitation: PBX1 Antibody [NBP2-19713] -

PBX1 antibody immunoprecipitates PBX1 protein in IP experiments.
IP Sample: 293T whole cell lysate/extract
A. Control with 2 ug of preimmune rabbit IgG
B. Immunoprecipitation of PBX1 protein by 2 ug of PBX1 antibody (NBP2-19713)
12 % SDS-PAGE
The immunoprecipitated PBX1 protein was detected by PBX1 antibody (NBP2-19713) diluted at 1:1000.
EasyBlot anti-rabbit IgG was used as a secondary reagent.

Applications for PBX1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PBX1

PBX1 binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development a

Alternate Names

DKFZp686B09108, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, Pre-B cell leukemia transcription factor-1, pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, PRL

Gene Symbol

PBX1

UniProt

P40424 (Human)

Additional PBX1 Products

Product Documents for PBX1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PBX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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