PCBP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-57878

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PCBP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ICAGGQDRCSDAAGYPHATHDLEGPPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCBP1 Antibody - BSA Free

Western Blot: PCBP1 Antibody [NBP2-57878]

Western Blot: PCBP1 Antibody [NBP2-57878]

Western Blot: PCBP1 Antibody [NBP2-57878] - Analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: PCBP1 Antibody [NBP2-57878]

Immunocytochemistry/ Immunofluorescence: PCBP1 Antibody [NBP2-57878]

Immunocytochemistry/Immunofluorescence: PCBP1 Antibody [NBP2-57878] - Staining of human cell line MCF7 shows localization to nuclear speckles & cytoplasmic bodies. Antibody staining is shown in green.
PCBP1 Antibody Western Blot: PCBP1 Antibody Antibody [NBP2-57878]

Western Blot: PCBP1 Antibody Antibody [NBP2-57878]

Analysis using NBP2-57878 (A) shows similar pattern to independent antibody NBP2-55063 (B).

Applications for PCBP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

1:100 - 1:250
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCBP1

PCBP1 is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq]

Alternate Names

Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein X, hnRNP E1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, nucleic acid binding protein sub 2.3, Nucleic acid-binding protein SUB2.3, poly(rC) binding protein 1, poly(rC)-binding protein 1

Gene Symbol

PCBP1

Additional PCBP1 Products

Product Documents for PCBP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PCBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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