PCNA Antibody (339) - Azide and BSA Free

Novus Biologicals | Catalog # NB100-478

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 339

Format

Azide and BSA Free
View all PCNA Primary Antibodies »

Product Specifications

Immunogen

Recombinant PCNA interdomain connector loop protein

Localization

Nuclear

Marker

Proliferation Marker

Specificity

This reacts with the interdomain connector loop of human PCNA, other not determined.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PCNA Antibody (339) - Azide and BSA Free

Western Blot: PCNA Antibody (339) [NB100-478]

Western Blot: PCNA Antibody (339) [NB100-478]

Western Blot: PCNA Antibody (339) [NB100-478] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (339) [NB100-478]

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (339) [NB100-478]

Immunocytochemistry/Immunofluorescence: PCNA Antibody (339) [NB100-478] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using 1:500 dilution.
Western Blot: PCNA Antibody (339) [NB100-478]

Western Blot: PCNA Antibody (339) [NB100-478]

Western Blot: PCNA Antibody (339) [NB100-478] - A: Jurkat, B: Raji, C: 293T, D: A431, E: HeLa, F: HepG2, G: H1299, H: HCT116, I: MCF-7, J: NT2D1, K: PC-3, L: U87-MG
Western Blot: PCNA Antibody (339) [NB100-478]

Western Blot: PCNA Antibody (339) [NB100-478]

Western Blot: PCNA Antibody (339) [NB100-478] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 12% SDS PAGE diluted at 1:1000

Applications for PCNA Antibody (339) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:500-1:1000
Application Notes
WB: Predicted MW is 31.32 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Entrez Gene IDs

5111 (Human)

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody (339) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PCNA Antibody (339) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PCNA Antibody (339) - Azide and BSA Free

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Protocols

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FAQs

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