PCNA Antibody (SY12-07)
Novus Biologicals | Catalog # NBP2-67390
Recombinant Monoclonal Antibody
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]](https://resources.rndsystems.com/images/products/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0010.jpg "Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SY12-07 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human PCNA aa 88-137 / 261. (SwissProt: P12004 Human; SwissProt: P17918 Mouse; SwissProt: P04961 Rat)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PCNA Antibody (SY12-07)
Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]
Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]](https://resources.rndsystems.com/images/products/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0007.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]")
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.![Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390]](https://resources.rndsystems.com/images/products/PCNA-Antibody-SY12-07-Flow-Cytometry-NBP2-67390-img0001.jpg "Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390]")
Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390]
Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]](https://resources.rndsystems.com/images/products/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0008.jpg "Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]")
Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]
Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]](https://resources.rndsystems.com/images/products/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0002.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]")
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]](https://resources.rndsystems.com/images/products/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0003.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]")
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]](https://resources.rndsystems.com/images/products/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0004.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]")
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]](https://resources.rndsystems.com/images/products/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0005.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]")
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]](https://resources.rndsystems.com/images/products/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0006.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]")
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.Applications for PCNA Antibody (SY12-07)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1:1000-1:5000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PCNA
Long Name
Proliferating Cell Nuclear Antigen
Alternate Names
cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Gene Symbol
PCNA
Additional PCNA Products
Product Documents for PCNA Antibody (SY12-07)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PCNA Antibody (SY12-07)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PCNA Antibody (SY12-07)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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