Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Rat

Predicted:

Bovine (100%), Chicken (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residues 75-125 of human proliferating cell nuclear antigen using the SwissProt entry P12004 (GeneID 5111).

Marker

Proliferation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNA Antibody - BSA Free

Western Blot: PCNA Antibody [NB100-456]

Western Blot: PCNA Antibody [NB100-456]

Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: PCNA Antibody [NB100-456]

Immunohistochemistry: PCNA Antibody [NB100-456]

Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB
Flow Cytometry: PCNA Antibody [NB100-456]

Flow Cytometry: PCNA Antibody [NB100-456]

Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA. Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.
Western Blot: PCNA Antibody [NB100-456]

Western Blot: PCNA Antibody [NB100-456]

Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates
Western Blot: PCNA Antibody [NB100-456]

Western Blot: PCNA Antibody [NB100-456]

Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation: PCNA Antibody [NB100-456]

Immunoprecipitation: PCNA Antibody [NB100-456]

Immunoprecipitation: PCNA Antibody [NB100-456] - Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody NB100-456 used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using another a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using NB100-456 (1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).
PCNA Antibody - BSA Free

Western Blot: PCNA Antibody - BSA Free [NB100-456] -

MLE and nCGA reduced the expression of proliferation-related proteins in diabetic cultured VSMCs. (A) The levels of PI3K, p-Akt, and PCNA in OH-treated A7r5 cells were notably increased under OH conditions and notably decreased after treatment with nCGA (40 and 120 μM). (B) In OH-stimulated A7r5 cells, the levels of Ras and p-ERK were increased, and OH-induced upregulation was nullified by nCGA. The quantitative data are presented as the mean +/- SD from a minimum of three independent experiments. # p < 0.05 as compared to the C (control). * p < 0.05 as compared to the OH group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35893859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PCNA Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PCNA

PCNA is a homotrimeric protein that is involved in DNA replication and DNA repair. It serves as a processivity factor for DNA polymerase delta. It has also been shown to interact with several other proteins that are involved in DNA replication and repair, including replication factor C, DNA ligase I, XPG, FEN1 and p21. This particular antiserum was raised against the interdomain connector loop region of PCNA, which is the region of PCNA through which many of the protein-protein interactions occur.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Entrez Gene IDs

5111 (Human)

Gene Symbol

PCNA

UniProt

Additional PCNA Products

Product Documents for PCNA Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PCNA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PCNA Antibody - BSA Free

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Protocols

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FAQs

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