PCNA Antibody

Western Blot: PCNA Antibody [NBP1-32075]

Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug NIH-3T3 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500

Immunocytochemistry/ Immunofluorescence: PCNA Antibody [NBP1-32075]

Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075]

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse testis. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075]

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human colon. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075]

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human cervical carcinoma. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: PCNA Antibody [NBP1-32075]

Immunoprecipitation: PCNA Antibody [NBP1-32075] - Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 ug of PCBP2 antibody. Western blot analysis was performed using PCBP2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: PCNA Antibody [NBP1-32075] -

Western Blot: PCNA Antibody [NBP1-32075] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (NBP1-32075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: PCNA Antibody [NBP1-32075] -

PCNA antibody detects PCNA protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (NBP1-32075) diluted at 1:2500.

Western Blot: PCNA Antibody [NBP1-32075] -

Activation (RAPA) (20 μM) and inhibition (CQ) (50 μM) of HIF-1 alpha affected autophagy, proliferation, and anti-apoptosis ability of yak heart CASMCs. (A–C) Effects of RAPA and CQ on the expression of autophagy-related proteins in yak heart CASMCs. (D–G) RAPA and CQ on the expression of LC3-II and P62 protein in yak heart CASMCs by IF analysis. Magnification: ×10, Bar = 200 μm. (H,I) The effects of RAPA and CQ on autophagy expression in yak heart CASMCs by Ad-mRFP-GFP-LC3B analysis. Bar = 20 μm. (J–L) The effects of RAPA and CQ on the expression of proliferation- and apoptosis-related proteins in yak heart CASMCs by Western blot. (M,O) EdU proliferation was used to detect the effect of RAPA and CQ on proliferation expression in yak heart CASMCs. (N,P) Tunel apoptosis was used to detect the effect of RAPA and CQ on apoptosis expression in yak heart CASMCs. Magnification: ×10, Bar = 200 μm. (** p < 0.01) (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40001559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PCNA Antibody [NBP1-32075] -

Hypoxia induces the expression of PCNA, Bax, and Bcl-2 in yak CASMCs. (A) Primary yak CASMCs were cultured in vitro. Magnification: ×10, Bar = 200 μm. (B) Identification of CASMCs in yak heart. Magnification: ×10, Bar = 200 μm. (C,D) Hypoxia-induced levels of PCNA, Bax, and Bcl-2 proteins in CASMCs of yak heart induced by Western blot. (E) Hypoxia-induced levels of PCNA, Bax, and Bcl-2 mRNAs in CASMCs of yak heart by qRT-PCR. (nsp ≥ 0.05, * p < 0.05, ** p < 0.01) (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40001559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PCNA Antibody [NBP1-32075] -

IHC, Western blot, and qRT-PCR analysis of changes in PCNA, Bax, and Bcl-2 in heart tissue of yak at different stages. (A) Localization and distribution of PCNA, Bax, and Bcl-2 proteins in yak hearts at different ages. Magnification: ×20, Bar = 100 μm. (B,C) The levels of PCNA, Bax, and Bcl-2 proteins in heart tissue of yaks at different ages were determined by Western blot. Western blot original images can be found in File S1. (D) The levels of PCNA, Bax, and Bcl-2 mRNAs in heart tissues of yaks at different ages were determined by qRT-PCR. (nsp ≥ 0.05, * p < 0.05, ** p < 0.01) (n = 3). ★: cardiomyocytes; ▲: vascular smooth muscle cells; ↑: vascular endothelial cells. Newborn: Newborn; 6 M: 6 months old; 2 Y: 2 years old; 4 Y: 4 years old; 6 Y: 6 years old; 10 Y: 10 years old. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40001559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PCNA Antibody [NBP1-32075] -

Activation (DMOG) (200 μM) and inhibition (LW6) (25 μM) of HIF-1 alpha affected the proliferation and anti-apoptotic ability of yak heart CASMCs. (A–C) Effects of DMOG and LW6 on the expression of proliferation- and apoptosis-related proteins in yak heart CASMCs. (D,F) EdU proliferation was used to detect the effect of DMOG and LW6 on the proliferation expression of yak heart CASMCs. (E,G) Tunel apoptosis assay was used to detect the effect of DMOG and LW6 on apoptosis expression in CASMCs of yak heart. Magnification: ×10, Bar = 200 μm. ( ** p < 0.01) (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40001559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.