PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10)
Novus Biologicals | Catalog # NBP2-46347
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI7B10
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Product Specifications
Immunogen
Full length human recombinant protein of human PDCD1LG2 (NP_079515) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
30.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10)
![Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Western-Blot-NBP2-46347-img0009.jpg "Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1LG2.![Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Immunohistochemistry-NBP2-46347-img0008.jpg "Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - Analysis of Carcinoma of Human liver tissue(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Flow-Cytometry-NBP2-46347-img0011.jpg "Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - analysis of living 293T cells transfected with PDL2 overexpression plasmid.![Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Immunohistochemistry-NBP2-46347-img0010.jpg "Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - PD-L2/B7-DC/PDCD1LG2 Antibody (7B10) [NBP2-46347] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Immunohistochemistry-NBP2-46347-img0001.jpg "Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Immunohistochemistry-NBP2-46347-img0002.jpg "Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Immunohistochemistry-NBP2-46347-img0003.jpg "Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Immunohistochemistry-NBP2-46347-img0004.jpg "Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Immunohistochemistry-NBP2-46347-img0006.jpg "Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]](https://resources.rndsystems.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-OTI7B10-Immunohistochemistry-NBP2-46347-img0007.jpg "Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]")
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347]
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10) [NBP2-46347] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)Applications for PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10)
Application
Recommended Usage
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PD-L2/B7-DC
Long Name
Programmed Death-1 Ligand-2
Alternate Names
B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2
Gene Symbol
PDCD1LG2
Additional PD-L2/B7-DC Products
Product Documents for PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PD-L2/B7-DC/PDCD1LG2 Antibody (OTI7B10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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