PDGFA Antibody (MM0013-6D35) - Azide and BSA Free

Novus Biologicals | Catalog # NB110-60979

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0013-6D35

Format

Azide and BSA Free
View all PDGF-A Primary Antibodies »

Product Specifications

Immunogen

Human recombinant PDGFA

Reactivity Notes

This antibody is reactive against human. Not yet tested in other species.

Specificity

In western blots this antibody shown no cross-reactivity with E-Cadherin, and P-Cadherin. There is 25% crossreactivitywith rhPDGF-AB, and no cross-reactivity with rhPDGF-BB.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Scientific Data Images for PDGFA Antibody (MM0013-6D35) - Azide and BSA Free

Western Blot: PDGFA Antibody (MM0013-6D35)Azide and BSA Free [NB110-60979]

Western Blot: PDGFA Antibody (MM0013-6D35)Azide and BSA Free [NB110-60979]

Western Blot: PDGFA Antibody (MM0013-6D35) - Azide and BSA Free [NB110-60979] - Lysate of human placenta tissue with mouse anti-human PDGFA antibody.
Immunohistochemistry-Paraffin: PDGFA Antibody (MM0013-6D35) - Azide and BSA Free [NB110-60979]

Immunohistochemistry-Paraffin: PDGFA Antibody (MM0013-6D35) - Azide and BSA Free [NB110-60979]

Immunohistochemistry-Paraffin: PDGFA Antibody (MM0013-6D35) [NB110-60979] - Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using NB110-60979 (Proteinase K pretreatment is required).

Applications for PDGFA Antibody (MM0013-6D35) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-A

PDGF AA is a member of the platelet derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGF AA can exist either as a homodimer or as a heterodimer with the platelet derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth.

Long Name

Platelet-derived Growth Factor A

Alternate Names

PDGF-1, PDGF1PDGF A-chain, PDGF-A, Platelet-derived growth factor A chain, platelet-derived growth factor alpha chain, platelet-derived growth factor alpha isoform 2 preproprotein, platelet-derived growth factor alpha polypeptidePDGF subunit A, platelet-derived growth factor subunit A

Entrez Gene IDs

5154 (Human)

Gene Symbol

PDGFA

Additional PDGF-A Products

Product Documents for PDGFA Antibody (MM0013-6D35) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PDGFA Antibody (MM0013-6D35) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

PDGF Family

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Protocols

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FAQs

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