PELP1 Antibody - BSA Free

Novus Biologicals | Catalog # NB200-331

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PELP1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide mapping to a region between residues 1000 and 1050 of human, proline-, glutamic acid-, leucine-rich protein 1/ modulator of nongenomic activity of estrogen receptor using the numbering given in entry AAN41255.1 (GeneID 27043).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PELP1 Antibody - BSA Free

Western Blot: PELP1 Antibody [NB200-331]

Western Blot: PELP1 Antibody [NB200-331]

Western Blot: PELP1 Antibody [NB200-331] - Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331]

Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331]

Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Human ovarian carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR used at a dilution of 1:2,500 (0.4ug/ml) (left) used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.
Western Blot: PELP1 Antibody [NB200-331]

Western Blot: PELP1 Antibody [NB200-331]

Western Blot: PELP1 Antibody [NB200-331] - Detection of Human PELP1/MNAR by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331]

Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331]

Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Samples: FFPE sections of mouse renal cell carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR dilution of 1:2,500 (0.4ug/ml) (left) and used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.
Immunoprecipitation: PELP1 Antibody [NB200-331]

Immunoprecipitation: PELP1 Antibody [NB200-331]

Immunoprecipitation: PELP1 Antibody [NB200-331] - Detection of human PELP1/MNAR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 (lot NB200-331-3) used for IP at 6 ug per reaction. PELP1/MNAR was also immunoprecipitated by a previous lot of this antibody (lot NB200-331-2) and rabbit anti-PELP1/MNAR antibody NB110-40622. For blotting immunoprecipitated PELP1/MNAR, NB200-331 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for PELP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2500-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PELP1

PELP1 is a coactivator of estrogen receptor (see ESR1; MIM 133430)-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (Choi et al., 2004).[supplied by OMIM]

Alternate Names

HMX3, MNARmodulator of nongenomic activity of estrogen receptor, Modulator of non-genomic activity of estrogen receptor, P160, proline and glutamic acid rich nuclear protein, proline, glutamate and leucine rich protein 1, proline, glutamic acid and leucine rich protein 1, proline-, glutamic acid- and leucine-rich protein 1, proline-, glutamic acid-, leucine-rich protein 1, Transcription factor HMX3

Entrez Gene IDs

27043 (Human)

Gene Symbol

PELP1

UniProt

AAN41255.1 (Human)

Additional PELP1 Products

Product Documents for PELP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PELP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PELP1 Antibody - BSA Free

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Protocols

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