Pepsin Antibody [Biotin]

Novus Biologicals | Catalog # NB600-614

Novus Biologicals

Key Product Details

Species Reactivity

Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Biotin

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach. (Uniprot: P00791)

Reactivity Notes

Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]

Scientific Data Images for Pepsin Antibody [Biotin]

Western Blot: Pepsin Antibody [Biotin] [NB600-614] - Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 41 kDa, 41 kDa for Pepsin.
Pepsin Antibody [Biotin]

Pepsin Antibody [Biotin]

Western Blot of Goat anti-Pepsin Antibody Biotin Conjugated. Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody [Biotin]1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin

Applications for Pepsin Antibody [Biotin]

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:2000
Application Notes
This product has been tested by ELISA and western. This product is suitable for Immunohistochemistry. Expect band at 41.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pepsin

Digestive enzyme with particularly broad specificity.

Alternate Names

DKFZp666J2410, ec 3.4.23.1, Pepsin a precursor, Pepsinogen 1 second locus, Pepsinogen 3 group 1, Pepsinogen 4 group I, Pepsinogen 5 group I, Pepsinogen A, Pepsinogen a3, Pepsinogen A4, Pepsinogen A5, Pepsinogen group 1, Pepsinogen I, Pga 3, Pga 4, Pga 5, pga3, pga4, pga5

Additional Pepsin Products

Product Documents for Pepsin Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Pepsin Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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