Peptide YY Antibody (4) - BSA Free

Novus Biologicals | Catalog # NBP2-23584

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4

Format

BSA Free
View all Peptide YY Primary Antibodies »

Product Specifications

Immunogen

Synthetic N-terminal fragment of human PYY(3-36) coupled to carrier.

Epitope

The epitope lies within residues 3-12 of human PYY

Specificity

Binds human PYY(3-36). Based on inhibition ELISA, shows no cross-reaction with PYY(1-36) or NPY.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for Peptide YY Antibody (4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-23584 binds free PYY(3-36) in solution. An ELISA dilution guideline of 1/20,000 applies to PYY(3-36) coated directly at 1 ug/mL. NBP2-23584 as a capture antibody forms a sandwich ELISA antibody pair with biotinylated NBP1-05167 as a detection antibody for measuring PYY(3-36). WB: NBP2-23584 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Peptide YY

Peptide tyrosine-tyrosine amide (3-36), or PYY(3-36), is a major metabolite of the gut hormone PYY and is produced by the action of dipeptidyl peptidase IV on PYY in the intestinal brush border and in the circulation. PYY(3-36) is a potent inhibitor of food intake in rats and humans, acting selectively on the Y2 receptor to inhibit orexigenic NPY neurons in the arcuate nucleus, thus disinhibiting anorexigenic POMC neurons. The anorexigenic effect of PYY(3-36) is additive to that of the co-secreted gut hormone GLP-1.

Alternate Names

Peptide-YY, PYY, PYY-I, PYY1

Gene Symbol

PYY

Additional Peptide YY Products

Product Documents for Peptide YY Antibody (4) - BSA Free

Certificate of Analysis

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Product Specific Notices for Peptide YY Antibody (4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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