PER1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24589

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Canine, Equine, Primate

Cited:

Mouse, Amphibian

Predicted:

Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Simple Western

Cited:

Knockout Validated, Western Blot, Immunoblotting, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PER1 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 1150-1200 of human PER1 was used as the immunogen.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29937374).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PER1 Antibody - BSA Free

Western Blot: PER1 Antibody [NBP2-24589]

Western Blot: PER1 Antibody [NBP2-24589]

Western Blot: PER1 Antibody [NBP2-24589] - Analysis of PER1 in HEPG2 cell lysates in the 1) absence and 2) presence of immunizing peptide using NBP2-24589. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: PER1 Antibody [NBP2-24589]

Immunohistochemistry-Paraffin: PER1 Antibody [NBP2-24589]

Immunohistochemistry-Paraffin: PER1 Antibody [NBP2-24589] - Human brain tissue using an isotype control (top) and NBP2-24589 (bottom) at 5 ug/ml.

Applications for PER1 Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 29937374)

Immunohistochemistry-Paraffin

5 ug/ml

Knockout Validated

reported in scientific literature (Hamilton et al)

Simple Western

1:1000 - 1:5000

Western Blot

5-10 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in C57BL/6 liver whole-cell lysate (CT15), separated by Size, antibody dilution of 1:1000, 1:2000, 1:5000, apparent MW was 146 kDa

Reviewed Applications

Read 2 reviews rated 1.5 using NBP2-24589 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PER1

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

Long Name

Period Circadian Protein 1

Alternate Names

PERIOD1, RIGUI

Gene Symbol

PER1

Additional PER1 Products

Product Documents for PER1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PER1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PER1 Antibody - BSA Free

Customer Reviews for PER1 Antibody - BSA Free (2)

1.5 out of 5
2 Customer Ratings
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Customer Images

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  • PER1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: brain section
    Species: Other
    Verified Customer | Posted 09/11/2023
    20um section including hypothalamus and SCN of Hyla cinerea. Staining using DAB
    PER1 Antibody - BSA Free NBP2-24589
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • PER1 Antibody
    Name: Katharina Sahiri
    Application: Western Blot
    Sample Tested: U-2 OS cells
    Species: Human
    Verified Customer | Posted 02/21/2019
    siRNA transfection optimisation: whole cell lysate of U-2 OS cells a) siRNA knockdown of PER1 (load 25 µg/well) b) wt U-2 OS cells with different protein amounts/well 1°AB over night, 4 °C 2°AB 1h, RT Nitrocellulose Membrane
    unspecific bands with no obvious band of correct size
    PER1 Antibody - BSA Free NBP2-24589

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