Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # B-D48

Format

Azide and BSA Free
View all Perforin Primary Antibodies »

Product Specifications

Immunogen

Natural human Perforin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Stable at 4C for 12 months.

Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells

Scientific Data Images for Perforin Antibody (B-D48) - Azide and BSA Free

Flow Cytometry: Perforin Antibody (B-D48) - Azide and BSA Free [NBP3-14581]

Flow Cytometry: Perforin Antibody (B-D48) - Azide and BSA Free [NBP3-14581]

Flow Cytometry: Perforin Antibody (B-D48) [NBP3-14581] - A typical staining pattern with the B-D48 monoclonal antibody of lymphocytes.

Applications for Perforin Antibody (B-D48) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Multiplex Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS. Sterile-filtered through 0.22 um.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Perforin

Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Gene Symbol

PRF1

Additional Perforin Products

Product Documents for Perforin Antibody (B-D48) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Perforin Antibody (B-D48) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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