Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25353

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2147 expressed in HEK293

Format

Azide and BSA Free
View all Perilipin-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragmemt of human Perilipin-2/ADFP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] -

Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (NBP3-25353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] -

Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (NBP3-25353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] -

Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] - ADFP antibody [HL2147] detects ADFP protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ADFP stained by ADFP antibody [HL2147] (NBP3-25353) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] -

Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (NBP3-25353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody (HL2147) [NBP3-25353] -

ADFP antibody [HL2147] detects ADFP protein at lipid droplet by immunofluorescent analysis.
Sample: Mock and differentiated 3T3-L1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ADFP stained by ADFP antibody [HL2147] (NBP3-25353) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Perilipin-2

Perilipin-2/Plin-2, also known as adipose differentiation-related protein (ADFP), ADRP, and adipophilin, is a member of the perilipin family of lipid droplet (LD) proteins that functions in lipid accumulation, lipid storage, and LD formation (1,2). Perilipin-2 is ubiquitously expressed and is the major LD protein of the liver (1-3). Human perilipin-2 is 437 amino acids (aa) in length with a theoretical molecular weight of 48 kDa (1,4). Human and mouse perilipin-2 share 81.8% sequence identity. One defining structural feature of perilipin-2 and other LD-related proteins is formation of amphipathic alpha helices (1,3,5). The perilipin-2 protein contains a PAT (perilipin A, ADRP, TIP47) domain, a 11-mer repeat motif, and a 4-helix bundle (1,5). The PAT domain has a role in triglyceride (TG) stabilization, the 11-mer repeat motif functions in cytoplasmic LD binding, and the 4-helix bundle facilitates membrane binding (5). Unlike LDs coated with other perilipin family members (e.g., perilipin-1 and perilipin-5), LDs coated with perilipin-2 are more permissive to lipolysis and protective of lipids and TGs (1-3). Studies have revealed that perilipin-2 knockout (KO) in mice is associated with resistance to fatty liver disease and diet-induced obesity (1). On the other hand, overexpression of perilipin-2 causes increased lipid and TG accumulation, which is a feature of many metabolic, cardiovascular, and age-related diseases (2). Additional research has found higher levels of perilipin-2 is associated with certain cancers such as colorectal cancer and renal carcinomas, and it may be a useful biomarker for detection (2). Though more research is needed, it is possible that inhibition or blocking of perilipin-2 may be a key strategy to combatting several pathologies (2).

References

1. Itabe H, Yamaguchi T, Nimura S, Sasabe N. Perilipins: a diversity of intracellular lipid droplet proteins. Lipids Health Dis. 2017;16(1):83. https://doi.org/10.1186/s12944-017-0473-y

2. Conte M, Franceschi C, Sandri M, Salvioli S. Perilipin 2 and Age-Related Metabolic Diseases: A New Perspective. Trends Endocrinol Metab. 2016;27(12):893-903. https://doi.org/10.1016/j.tem.2016.09.001

3. Sztalryd C, Brasaemle DL. The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862(10 Pt B):1221-1232. https://doi.org/10.1016/j.bbalip.2017.07.009

4. Uniprot (Q99541)

5. Chong BM, Reigan P, Mayle-Combs KD, Orlicky DJ, McManaman JL. Determinants of adipophilin function in milk lipid formation and secretion. Trends Endocrinol Metab. 2011;22(6):211-217. https://10.1016/j.tem.2011.04.003

Alternate Names

ADFP, Adipophilin, Perilipin2, PLIN2

Gene Symbol

PLIN2

Additional Perilipin-2 Products

Product Documents for Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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