Periostin/OSF-2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-88950

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Predicted:

Amphibian (%), Avian (%), Bovine (100%), Canine (100%), Equine (100%), Feline (100%), Finch (100%), Fish (%), Goat (100%), Guinea Pig (100%), Hamster (100%), Primate (%), Rabbit (100%), Sheep (100%), Vole (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Periostin/OSF-2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the fasciclin domain 1 of Periostin/OSF-2. Accession # Q62009

Reactivity Notes

Expected reactivity with Avian, Amphibians, Fish, and most mammals.

Specificity

Specific for endogenous levels of the three Periostin/OSF-2 bands around ~93 kDa. The immunogenic peptide is present in all known splice variants of Periostin/OSF-2 and therefore this antibody recognizes all known molecular forms of Periostin/OSF-2 and is thus pan-specific.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for Periostin/OSF-2 Antibody - Azide and BSA Free

Western Blot: Periostin/OSF-2 AntibodyAzide and BSA Free [NBP2-88950]

Western Blot: Periostin/OSF-2 AntibodyAzide and BSA Free [NBP2-88950]

Western Blot: Periostin/OSF-2 - Western blot of rat lung lysate showing specific immunolabeling of the ~93 kDa Periostin/OSF-2 protein triplet.

Applications for Periostin/OSF-2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Western Blot

1:1000
Application Notes
This Periostin/OSF-2 Antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Gene Symbol

POSTN

Additional Periostin/OSF-2 Products

Product Documents for Periostin/OSF-2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Periostin/OSF-2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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