Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PERQ2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 375 and 425 of human GRB10 Interacting GYF protein 2 using the numbering given in entry NP_001096616.1 (GeneID 26058).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PERQ2 Antibody - BSA Free

Immunoprecipitation: PERQ2 Antibody [NBP2-12812]

Immunoprecipitation: PERQ2 Antibody [NBP2-12812]

Immunoprecipitation: PERQ2 Antibody [NBP2-12812] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PERQ2 antibody NBP2-12811 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PERQ2 was also immunoprecipitated by rabbit anti-PERQ2 antibodies NBP2-12812 and NBP2-12813, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Applications for PERQ2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 28585547).

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 3 reviews rated 3.7 using NBP2-12812 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PERQ2

Alternate Names

DKFZp686J17223, FLJ23368, GRB10 interacting GYF protein 2, GRB10-interacting GYF protein 2, GYF domain containing 2, GYF2, KIAA0642DKFZp686I15154, PARK11, Parkinson disease (autosomal recessive, early onset) 11, PERQ amino acid rich, with GYF domain 2, PERQ amino acid rich, with GYF domain 3, PERQ amino acid-rich with GYF domain-containing protein 2, PERQ2, PERQ3, TNRC15, trinucleotide repeat containing 15, Trinucleotide repeat-containing gene 15 protein

Gene Symbol

GIGYF2

UniProt

NP_001096616.1 (Human)

Additional PERQ2 Products

Product Documents for PERQ2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PERQ2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PERQ2 Antibody - BSA Free

Customer Reviews for PERQ2 Antibody - BSA Free (3)

3.7 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • PERQ2 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: b-cell
    Species: Human
    Verified Customer | Posted 01/02/2019
    PERQ2 immunoprecipitation in human B-cells.
    5ug antibody used with 500ug whole cell lysate. L1: 40ug input, L2: IgG, L3: IP (10ul of 50ul elution)
    PERQ2 Antibody - BSA Free NBP2-12812
  • PERQ2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 11/18/2017
    PERQ2 in fibroblasts
    Blocked 1H 25C in 1X PBS/5% normal serum/0.3% Triton™ X-100 Diluted 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation. 2 hours (25C) secondary (1.5:1000 dilution)
    PERQ2 Antibody - BSA Free NBP2-12812
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: human lymphocytes, whole cell lysate
    Species: Human
    Verified Customer | Posted 04/15/2016
    PERQ2 in Human Lymphocyte lysate
    PERQ2 Antibody - BSA Free NBP2-12812

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