PFKFB4 Antibody (HL2204) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25598
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2204 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of human PFKFB4.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PFKFB4 Antibody (HL2204) - Azide and BSA Free
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] -
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] -
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] -
Immunohistochemistry-Paraffin: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. PFKFB4 stained by PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] -
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] -
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] -
Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] -
Immunohistochemistry-Paraffin: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. PFKFB4 stained by PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: PFKFB4 Antibody (HL2204) [NBP3-25598] -
PFKFB4 antibody [HL2204] detects PFKFB4 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.
Green: PFKFB4 stained by PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Applications for PFKFB4 Antibody (HL2204) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PFKFB4
Long Name
6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase 4
Alternate Names
6PF-2-K/Fru-2,6-P2ase 4, PFK/FBPase 4
Gene Symbol
PFKFB4
Additional PFKFB4 Products
Product Documents for PFKFB4 Antibody (HL2204) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PFKFB4 Antibody (HL2204) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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