PGAM1 Antibody (7V9H9)

Name: PGAM1 Antibody (7V9H9)
Brand: Novus Biologicals
SKU: NBP3-16347
Price: 179 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16347

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7V9H9 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PGAM1 (P18669). MAAYKLVLIRHGESAWNLENRFSGWYDADLSPAGHEEAKRGGQALRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PGAM1 Antibody (7V9H9)

Western Blot: PGAM1 Antibody (7V9H9) [NBP3-16347]

Western Blot: PGAM1 Antibody (7V9H9) [NBP3-16347] - Western blot analysis of extracts of various cell lines, using PGAM1 Rabbit mAb (NBP3-16347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: PGAM1 Antibody (7V9H9) [NBP3-16347]

Immunohistochemistry-Paraffin: PGAM1 Antibody (7V9H9) [NBP3-16347] - Immunohistochemistry of paraffin-embedded human appendix using PGAM1 Rabbit mAb (NBP3-16347) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for PGAM1 Antibody (7V9H9)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PGAM1

PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia.

Alternate Names

BPG-dependent PGAM 1, EC 3.1.3.13, EC 5.4.2.1, EC 5.4.2.4, PGAMA, PGAM-Bphosphoglycerate mutase 1, phosphoglycerate mutase 1 (brain), phosphoglycerate mutase A, nonmuscle form, Phosphoglycerate mutase isozyme B

Gene Symbol

PGAM1

Additional PGAM1 Products

Product Documents for PGAM1 Antibody (7V9H9)

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Certificate of Analysis

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Product Specific Notices for PGAM1 Antibody (7V9H9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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