PGC-1 beta Antibody (6C3F6) - BSA Free

Novus Biologicals | Catalog # NBP2-37249

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6C3F6

Format

BSA Free
View all PGC1 beta Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PGC-1 beta (AA: 195-414) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

113.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGC-1 beta Antibody (6C3F6) - BSA Free

Western Blot: PGC-1 beta Antibody (6C3F6)BSA Free [NBP2-37249]

Western Blot: PGC-1 beta Antibody (6C3F6)BSA Free [NBP2-37249]

Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against HEK293 (1) and PPARGC1B (AA: 195-414)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: PGC-1 beta Antibody (6C3F6) - BSA Free [NBP2-37249]

Flow Cytometry: PGC-1 beta Antibody (6C3F6) - BSA Free [NBP2-37249]

Flow Cytometry: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Flow cytometric analysis of Hela cells using PPARGC1B mouse mAb (green) and negative control (red).
Western Blot: PGC-1 beta Antibody (6C3F6)BSA Free [NBP2-37249]

Western Blot: PGC-1 beta Antibody (6C3F6)BSA Free [NBP2-37249]

Western Blot: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Western blot analysis using PPARGC1B mAb against human PPARGC1B (AA: 195-414) recombinant protein. (Expected MW is 50.3 kDa)
ELISA: PGC-1 beta Antibody (6C3F6) - BSA Free [NBP2-37249]

ELISA: PGC-1 beta Antibody (6C3F6) - BSA Free [NBP2-37249]

ELISA: PGC-1 beta Antibody (6C3F6) [NBP2-37249] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PGC-1 beta Antibody (6C3F6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGC1 beta

PGC-1 beta is a tissue specific co-activator that enhances the activity of estrogen receptor alpha. The co-activation function relies on a bipartite transcriptional activation domain and two LXXLL motifs that interact with the AR2 domain of ER-alpha in an estrogen-dependent manner. PGC-1 beta is also involved in activation of nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. Ectopic expression of this protein has been shown to lead to increased mitochondrial numbers and basal oxygen consumption.

Long Name

Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 beta

Alternate Names

ERRL1, PERC, PPARGC1B

Gene Symbol

PPARGC1B

Additional PGC1 beta Products

Product Documents for PGC-1 beta Antibody (6C3F6) - BSA Free

Certificate of Analysis

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Product Specific Notices for PGC-1 beta Antibody (6C3F6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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