Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A5

Format

Azide and BSA Free
View all 6-Phosphogluconate Dehydrogenase/PGD Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PGD(NP_002622) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGD Antibody (OTI2A5) - Azide and BSA Free

Western Blot: PGD Antibody (OTI2A5)Azide and BSA Free [NBP2-73361]

Western Blot: PGD Antibody (OTI2A5)Azide and BSA Free [NBP2-73361]

Western Blot: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGD.
Immunocytochemistry/ Immunofluorescence: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunocytochemistry/ Immunofluorescence: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunocytochemistry/Immunofluorescence: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PGD.
Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Human thyroid tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Carcinoma of Human prostate tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Human breast tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Human endometrium tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Human Kidney tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Human ovary tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Human pancreas tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361]

Immunohistochemistry: PGD Antibody (OTI2A5) - Azide and BSA Free [NBP2-73361] - Human prostate tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Applications for PGD Antibody (OTI2A5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 6-Phosphogluconate Dehydrogenase/PGD

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies.

Long Name

6-Phosphogluconate Dehydrogenase

Alternate Names

6PGD, PGDH

Gene Symbol

PGD

Additional 6-Phosphogluconate Dehydrogenase/PGD Products

Product Documents for PGD Antibody (OTI2A5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PGD Antibody (OTI2A5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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