PGD Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31589

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (94%), Chicken (90%), Chimpanzee (90%), Rat (95%), Sheep (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 6-Phosphogluconate Dehydrogenase/PGD Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGD. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (85%) and Zebrafish (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PGD Antibody - BSA Free (NBP1-31589) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PGD Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PGD Antibody - BSA Free

Western Blot: PGD Antibody [NBP1-31589]

Western Blot: PGD Antibody [NBP1-31589]

Western Blot: PGD Antibody [NBP1-31589] - Sample(30 ug whole cell lysate)A:293T B:MOLT4 C:Raji 10% SDS PAGE, antibody diluted at 1:1000.
PGD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PGD Antibody [NBP1-31589] -

PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGD protein stained by PGD antibody [N1N3] (NBP1-31589) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.
PGD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PGD Antibody [NBP1-31589] -

PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PGD protein stained by PGD antibody [N1N3] (NBP1-31589) diluted at 1:500.
Blue: Hoechst 33342 staining.
PGD Antibody - BSA Free

Western Blot: PGD Antibody [NBP1-31589] -

PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (NBP1-31589) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PGD Antibody - BSA Free

Immunohistochemistry: PGD Antibody [NBP1-31589] -

PGD antibody [N1N3] detects PGD protein at cytoplasm on A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
PGD antibody [N1N3] (NBP1-31589) dilution: 1:500.

Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
PGD Antibody - BSA Free

Western Blot: PGD Antibody [NBP1-31589] -

PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (NBP1-31589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PGD Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 6-Phosphogluconate Dehydrogenase/PGD

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies.

Long Name

6-Phosphogluconate Dehydrogenase

Alternate Names

6PGD, PGDH

Gene Symbol

PGD

Additional 6-Phosphogluconate Dehydrogenase/PGD Products

Product Documents for PGD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PGD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for PGD Antibody - BSA Free

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Protocols

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