PGK1 Antibody

Novus Biologicals | Catalog # NBP1-33685

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (97%), Porcine (96%), Primate (100%), Rhesus Macaque (100%), Sheep (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PGK1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%), Zebrafish (87%), Xenopus laevis (85%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGK1 Antibody

Immunohistochemistry-Paraffin: PGK1 Antibody [NBP1-33685]

Immunohistochemistry-Paraffin: PGK1 Antibody [NBP1-33685]

Immunohistochemistry-Paraffin: PGK1 Antibody [NBP1-33685] - PGK1 antibody detects PGK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PGK1 stained by PGK1 antibody diluted at 1:500.
Western Blot: PGK1 Antibody [NBP1-33685]

Western Blot: PGK1 Antibody [NBP1-33685]

Western Blot: PGK1 Antibody [NBP1-33685] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: PGK1 Antibody [NBP1-33685]

Western Blot: PGK1 Antibody [NBP1-33685]

Western Blot: PGK1 Antibody [NBP1-33685] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:100
Immunohistochemistry-Paraffin: PGK1 Antibody [NBP1-33685]

Immunohistochemistry-Paraffin: PGK1 Antibody [NBP1-33685]

Immunohistochemistry-Paraffin: PGK1 Antibody [NBP1-33685] - PGK1 antibody detects PGK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PGK1 stained by PGK1 antibody diluted at 1:500.
PGK1 Antibody

Western Blot: PGK1 Antibody [NBP1-33685] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [N1C1] (NBP1-33685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PGK1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PGK1

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Long Name

Phosphoglycerate Kinase-1

Alternate Names

MIG10, PGKA, PRP2

Gene Symbol

PGK1

Additional PGK1 Products

Product Documents for PGK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PGK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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