PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80909

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 188C424

Format

Azide and BSA Free
View all PGLYRP1/PGRP-S Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 165-180 (YVLKGHRDVQRTLSPG) of human PGRP-S (NP_005082).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Theoretical MW

21.73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free

Western Blot: PGLYRP1/PGRP-S Antibody (188C424)Azide and BSA Free [NBP2-80909]

Western Blot: PGLYRP1/PGRP-S Antibody (188C424)Azide and BSA Free [NBP2-80909]

Western Blot: PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free [NBP2-80909] - Analysis using PGLYRP1/PGRP-S antibody. Lysate from human Jurkat cells probed with PGRP-S antibody at 2 ug/ml. Image from the standard format of this antibody.
Immunohistochemistry: PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free [NBP2-80909]

Immunohistochemistry: PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free [NBP2-80909]

Immunohistochemistry: PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free [NBP2-80909] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human esophagus using PGLYRPI/PGRP-S antibody at 20 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate

Applications for PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 15839897)

Flow (Intracellular)

reported in scientific literature (PMID 19666596)

Flow Cytometry

1:20-1:20000

Immunocytochemistry/ Immunofluorescence

1:20-1:1000. Use reported in scientific literature (Dukhanina et al (2009))

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Frozen

1:20-1:1000. Use reported in scientific literature (Onkelen et al (2012))

Western Blot

2 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGLYRP1/PGRP-S

The primary immune recognition is based on structures common among invading pathogens. Bacterial surface molecules, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), are known to elicit immune reactions ranging from cytokine release to fever. Recently, a family of proteins called peptidoglycan recognition protein (PGRP) has been identified in mouse and human that binds to peptidoglycans expressed on Gram-positive bacteria. Peptidoglycan (PGN) is an essential cell wall component of virtually all bacteria and, thus, it is an excellent target for recognition by the eukaryotic innate immune system. The PGRPs (PGRP-L, PGRP-S, PGRP-Ia, and PGRP-Ib) define a new family of human pattern recognition molecules. PGRP-L is primarily expressed in the liver. Although liver is not considered a primary immune organ, liver participates in host defenses by producing acute phase proteins (by hepatocytes) in response to infections and by clearing microorganisms from blood. PGRP-S is present in neutrophils and inhibits growth of Gram-positive bacteria and, therefore, may function as a neutrophil antibacterial protein. However, PGRP-S may have another, as yet unidentified function because in humans it is expressed in the bone marrow 50-100 times higher than in neutrophils.

Long Name

Peptidoglycan Recognition Protein Short/Peptidoglycan Recognition Protein 1

Alternate Names

PGRP-S, PGRPS, TAG7, TNFSF3L

Gene Symbol

PGLYRP1

Additional PGLYRP1/PGRP-S Products

Product Documents for PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Host Defense/Innate Immunity

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Protocols

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