PGRMC1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-83220
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.rndsystems.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0020.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DQPAASGDSDDDEPPPLPRLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PGRMC1 Antibody - BSA Free
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.rndsystems.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0021.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular and stromal cells.![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.rndsystems.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0022.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.rndsystems.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0023.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.rndsystems.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0024.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.rndsystems.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0025.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.![Simple Western: PGRMC1 Antibody [NBP1-83220]](https://resources.rndsystems.com/images/products/PGRMC1-Antibody-Simple-Western-NBP1-83220-img0005.jpg "Simple Western: PGRMC1 Antibody [NBP1-83220]")
Simple Western: PGRMC1 Antibody [NBP1-83220]
Simple Western: PGRMC1 Antibody [NBP1-83220] - Simple Western lane view shows a specific band for PGRMC1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: PGRMC1 Antibody [NBP1-83220]](https://resources.rndsystems.com/images/products/PGRMC1-Antibody-Simple-Western-NBP1-83220-img0006.jpg "Simple Western: PGRMC1 Antibody [NBP1-83220]")
Simple Western: PGRMC1 Antibody [NBP1-83220]
Simple Western: PGRMC1 Antibody [NBP1-83220] - Electropherogram image(s) of corresponding Simple Western lane view. PGRMC1 antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).![PGRMC1 Antibody - BSA Free Immunohistochemistry-Paraffin: PGRMC1 Antibody - BSA Free [NBP1-83220]](https://resources.rndsystems.com/images/products/nbp1-83220_rabbit-polyclonal-pgrmc1-antibody-14202513424838.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody - BSA Free [NBP1-83220]")
Immunohistochemistry-Paraffin: PGRMC1 Antibody - BSA Free [NBP1-83220]
Analysis in human liver and skeletal muscle tissues using NBP1-83220 antibody. Corresponding PGRMC1 RNA-seq data are presented for the same tissues.![PGRMC1 Antibody - BSA Free Western Blot: PGRMC1 Antibody - BSA Free [NBP1-83220]](https://resources.rndsystems.com/images/products/nbp1-83220_rabbit-polyclonal-pgrmc1-antibody-84202517254716.jpg "Western Blot: PGRMC1 Antibody - BSA Free [NBP1-83220]")
Western Blot: PGRMC1 Antibody - BSA Free [NBP1-83220]
Analysis in human liver tissue.![PGRMC1 Antibody - BSA Free Western Blot: PGRMC1 Antibody - BSA Free [NBP1-83220]](https://resources.rndsystems.com/images/products/nbp1-83220_rabbit-polyclonal-pgrmc1-antibody-8420251775474.jpg "Western Blot: PGRMC1 Antibody - BSA Free [NBP1-83220]")
Western Blot: PGRMC1 Antibody - BSA Free [NBP1-83220]
Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![PGRMC1 Antibody - BSA Free Western Blot: PGRMC1 Antibody - BSA Free [NBP1-83220]](https://resources.rndsystems.com/images/products/nbp1-83220_rabbit-polyclonal-pgrmc1-antibody-8420251720242.jpg "Western Blot: PGRMC1 Antibody - BSA Free [NBP1-83220]")
Western Blot: PGRMC1 Antibody - BSA Free [NBP1-83220]
Analysis in human cell line HEK 293.Applications for PGRMC1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500-1:1000
Simple Western
1:20
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 35 kDa
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PGRMC1
Long Name
Progesterone Receptor Membrane Component 1
Alternate Names
HPR6.6, MPR, Progesterone Binding Protein
Gene Symbol
PGRMC1
Additional PGRMC1 Products
Product Documents for PGRMC1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PGRMC1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PGRMC1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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